gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory.
gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory.
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DOI：
10.1084/jem.180.1.157
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发表时间：
1994-07-01
影响因子：
15.3
通讯作者：
Noelle, Randolph J.
中科院分区：
文献类型：
--
作者：
Foy, Teresa M.;Laman, Jon D.;Ledbetter, Jeffrey A.;Aruffo, Alejandro;Claassen, Eric;Noelle, Randolph J.
gp 39是在活化的CD 4 + T辅助细胞上表达的CD 40的配体，是产生对T依赖性（TD）抗原的抗体应答所必需的。用抗gp 39在体内处理小鼠抑制TD的一抗和二抗形成，但不抑制T非依赖性抗原。然而，这种受体-配体对在生发中心的发育和B细胞记忆的产生中的作用尚未确定。使用抗体的gp 39，本研究探讨在体内的gp 39-CD 40的相互作用，在诱导生发中心的形成，以及在产生B细胞记忆的要求。免疫动物，在体内用抗gp 39处理，并在免疫后9-11天使用免疫组织化学染色评估脾生发中心的存在。结果表明，作为用抗gp 39处理的结果，生发中心的形成被完全抑制。此外，过继转移实验表明，由于阻断gp 39-CD 40相互作用，抗原特异性记忆B细胞的产生也受到抑制.综上所述，数据表明，gp 39-CD 40相互作用不仅对于抗体应答的产生，而且对于B细胞记忆的发展是关键的。
gp39, the ligand for CD40 expressed on activated CD4+ T helper cells, is required for the generation of antibody responses to T-dependent (TD) antigens. Treatment of mice with anti-gp39 in vivo inhibits both primary and secondary antibody formation to TD, but not T-independent antigens. However, the role of this receptor-ligand pair in the development of germinal centers and the generation of B cell memory is as yet undefined. Using an antibody to gp39, this study examines the in vivo requirement for gp39-CD40 interactions in the induction of germinal center formation, as well as in the generation of B cell memory. Animals were immunized, treated in vivo with anti-gp39, and evaluated using immunohistochemical staining for the presence of splenic germinal centers 9-11 d after immunization. The results demonstrate that the formation of germinal centers was completely inhibited as a result of treatment with anti-gp39. Moreover, adoptive transfer experiments demonstrate that the generation of antigen- specific memory B cells is also inhibited as a consequence of blocking gp39-CD40 interactions. Taken together, the data demonstrate that gp39- CD40 interactions are critical not only for the generation of antibody responses, but also in the development of B cell memory.
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影响因子：
56.9
作者：
BANCHEREAU, J;DEPAOLI, P;ROUSSET, F
通讯作者：
ROUSSET, F
DOI：
10.1084/jem.179.3.819
发表时间：
1994-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Lane P;Burdet C;Hubele S;Scheidegger D;Müller U;McConnell F;Kosco-Vilbois M
通讯作者：
Kosco-Vilbois M
影响因子：
15.3
作者：
Alderson, M R;Armitage, R J;Tough, T W;Strockbine, L;Fanslow, W C;Spriggs, M K
通讯作者：
Spriggs, M K
DOI：
10.1084/jem.178.5.1567
发表时间：
1993-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Foy TM;Shepherd DM;Durie FH;Aruffo A;Ledbetter JA;Noelle RJ
通讯作者：
Noelle RJ
影响因子：
64.8
作者：
BANCHEREAU, J;ROUSSET, F
通讯作者：
ROUSSET, F