Pim-3 is a Critical Risk Factor in Development and Prognosis of Prostate Cancer.
Pim-3 is a Critical Risk Factor in Development and Prognosis of Prostate Cancer.
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Pim-3 是前列腺癌发生和预后的关键危险因素
DOI：
10.12659/msm.898223
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发表时间：
2016-11-09
期刊：
影响因子：
--
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Qu Y;Zhang C;Du E;Wang A;Yang Y;Guo J;Wang A;Zhang Z;Xu Y
Pim-3激酶是一种高度同源的丝氨酸/苏氨酸激酶，在恶性血液病和实体瘤中过度表达。很少有研究确定Pim-3在实体瘤中的作用，特别是在前列腺癌中。本研究的目的是确定Pim-3在前列腺癌的发展和预后中的作用。材料/方法收集160例前列腺癌患者和100例前列腺增生患者的前列腺组织标本。实时聚合酶链反应用于在RNA水平上评估Pim-3表达，并且蛋白质印迹用于定量3种不同细胞系中的Pim-3蛋白合成。结果前列腺癌组织中Pim-3 mRNA的表达明显高于良性前列腺增生组织（p<0.05）。因此，前列腺癌细胞系中Pim-3的蛋白水平表达也显著高于对照细胞。此外，Pim-3 mRNA的表达状态与前列腺特异性抗原、Gleason评分、病理分期、淋巴结转移等病理参数显著相关。Pim-3的高表达也显著降低了患者术后的生存率。结论Pim-3表达是前列腺癌的重要危险因素，我们是第一个在中国人中报道Pim-3作为一个有价值的生物标志物的团队。
Background Pim-3 kinase is a highly homologous serine/threonine kinase that is overexpressed in hematological malignancies and solid tumors. Few studies have been conducted to define the role of Pim-3 in solid tumors, especially in prostate cancer. The aim of this study was to define the role of Pim-3 in development and prognosis of prostate cancer. Material/Methods We collected specimens from 160 patients with prostate cancer, as well as 100 patients with benign prostatic hyperplasia. Realtime polymerase chain reaction was used for the assessment of Pim-3 expression at the RNA level and Western blot was used to quantify the Pim-3 protein synthesis in 3 different cell lines. Results We found that Pim-3 mRNA expression in prostate cancer tissue was significantly higher than that in benign prostatic hyperplasia tissue (p<0.05). Accordingly, the protein level expression of Pim-3 in prostate cancer cell lines was also significantly higher than that in control cells. In addition, the expression status of Pim-3 mRNA was significantly associated with pathological parameters such as pre-surgery prostate specific antigen, Gleason score, pathological stage, and lymphoid metastasis. High expression of Pim-3 also significantly decreased the survival rate of patients after surgery. Conclusions Pim-3 expression is an important risk factor for prostate cancer; we are the first team to report Pim-3 as a valuable biomarker in Chinese.
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