Systematic review of Plasmodium falciparum and Plasmodium vivax polyclonal infections: Impact of prevalence, study population characteristics, and laboratory procedures.
Systematic review of Plasmodium falciparum and Plasmodium vivax polyclonal infections: Impact of prevalence, study population characteristics, and laboratory procedures.
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DOI：
10.1371/journal.pone.0249382
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Koepfli C
中科院分区：
文献类型：
--
作者：
Lopez L;Koepfli C
在许多疟疾流行环境中，同一疟原虫物种的遗传上不同的克隆的多重感染是常见的。平均感染复数（MOI）和多克隆感染的比例通常被报道为传播强度的替代标志物，但与寄生虫流行率等传统指标的关系尚不清楚。我们在Pubmed上搜索了关于恶性疟原虫和间日疟原虫多重性的文章，并比较了多克隆感染的比例和平均MOI与人群患病率。评估了基因分型方法、基因分型标志物数量、诊断方法（显微镜/RDT vs. PCR）、临床症状的存在、年龄、地理区域和样本采集年份对多重性指数的影响。对于恶性疟原虫，153项研究符合纳入标准，产生了275个个体数据点和33，526个基因型个体。多克隆感染的比例范围为0- 96%，平均MOI为1-6.1。对于间日疟原虫，54项研究符合纳入标准，产生了115个数据点和13，325个基因型个体。多克隆感染的比例范围为0- 100%，平均MOI范围为1-3.8。对于这两个物种，多克隆感染的比例范围从非常低到接近100%，在低流行率，而在高流行率，它总是很高。恶性疟原虫感染率每增加一个百分点，多克隆感染率增加0.34%（P<0.001），多克隆间日疟原虫感染率增加0.78%（P<0.001）。在多变量分析中，发现较高的患病率、多种标记物分型、通过PCR诊断感染以及在非洲采样导致恶性疟原虫多克隆感染的比例较高。对于间日疟原虫，患病率、研究年份、分型多个标记物和地理区域是显著的预测因子。总之，多克隆感染经常出现在所有环境中，但多重性和患病率之间的关联很弱。
Multiple infections of genetically distinct clones of the same Plasmodium species are common in many malaria endemic settings. Mean multiplicity of infection (MOI) and the proportion of polyclonal infections are often reported as surrogate marker of transmission intensity, yet the relationship with traditional measures such as parasite prevalence is not well understood. We have searched Pubmed for articles on P. falciparum and P. vivax multiplicity, and compared the proportion of polyclonal infections and mean MOI to population prevalence. The impact of the genotyping method, number of genotyping markers, method for diagnosis (microscopy/RDT vs. PCR), presence of clinical symptoms, age, geographic region, and year of sample collection on multiplicity indices were assessed. For P. falciparum, 153 studies met inclusion criteria, yielding 275 individual data points and 33,526 genotyped individuals. The proportion of polyclonal infections ranged from 0–96%, and mean MOI from 1–6.1. For P. vivax, 54 studies met inclusion criteria, yielding 115 data points and 13,325 genotyped individuals. The proportion of polyclonal infections ranged from 0–100%, and mean MOI from 1–3.8. For both species, the proportion of polyclonal infections ranged from very low to close to 100% at low prevalence, while at high prevalence it was always high. Each percentage point increase in prevalence resulted in a 0.34% increase in the proportion of polyclonal P. falciparum infections (P<0.001), and a 0.78% increase in the proportion of polyclonal P. vivax infections (P<0.001). In multivariable analysis, higher prevalence, typing multiple markers, diagnosis of infections by PCR, and sampling in Africa were found to result in a higher proportion of P. falciparum polyclonal infections. For P. vivax, prevalence, year of study, typing multiple markers, and geographic region were significant predictors. In conclusion, polyclonal infections are frequently present in all settings, but the association between multiplicity and prevalence is weak.
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影响因子：
4.4
作者：
Lerch A;Koepfli C;Hofmann NE;Messerli C;Wilcox S;Kattenberg JH;Betuela I;O'Connor L;Mueller I;Felger I
通讯作者：
Felger I
DOI：
10.1016/j.meegid.2018.08.018
发表时间：
2018-11
期刊：
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
影响因子：
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作者：
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通讯作者：
Ward SA
DOI：
10.1016/s0035-9203(99)90322-x
发表时间：
1999-02-01
影响因子：
2.2
作者：
Smith, T;Beck, HP;Tanner, M
通讯作者：
Tanner, M
DOI：
10.1111/j.1471-8286.2006.01534.x
发表时间：
2007-01-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
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作者：
Karunaweera, N. D.;Ferreira, M. . U.;Wirth, D. F.
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Wirth, D. F.
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影响因子：
3
作者：
Cheng Q;Gatton ML;Barnwell J;Chiodini P;McCarthy J;Bell D;Cunningham J
通讯作者：
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