Preparation of endostatin-loaded chitosan nanoparticles and evaluation of the antitumor effect of such nanoparticles on the Lewis lung cancer model.
Preparation of endostatin-loaded chitosan nanoparticles and evaluation of the antitumor effect of such nanoparticles on the Lewis lung cancer model.
复制标题
内皮抑素壳聚糖纳米粒的制备及其对Lewis肺癌模型的抗肿瘤作用评价。
DOI：
10.1080/10717544.2016.1247927
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发表时间：
2017-11
期刊：
影响因子：
6
通讯作者：
Wen QL
中科院分区：
文献类型：
--
作者：
Ding RL;Xie F;Hu Y;Fu SZ;Wu JB;Fan J;He WF;He Y;Yang LL;Lin S;Wen QL
本研究的目的是制备ES-壳聚糖纳米粒（ES-NPs）并评价其对刘易斯肺癌模型的抗肿瘤作用。通过简单的离子交联方法制备ES-NP。进行ES-NP的表征，包括尺寸分布、ζ电位、负载效率和包封效率（EE）。体外释放试验也用于确定ES-NP的释放行为。测定细胞活力和细胞迁移以检测ES-NP的体外抗血管生成作用。为了阐明ES-NP在体内的抗肿瘤作用，使用刘易斯肺癌模型。结果表明，所制备的ES-NPs具有良好的粒径分布和高的EE，纳米粒呈球形，形态均一，体外释放良好。此外，ES-NPs显着抑制人脐静脉内皮细胞（HUVECs）的增殖和迁移。通过ELISA和免疫组织化学分析评价体内抗血管生成活性，这表明ES-NPs具有更强的抗血管生成作用，增强了抗癌活性。事实上，即使每7天注射一次ES-NP的治疗周期也显示出比连续14天注射游离ES更强的抗肿瘤效果。我们的研究证实CS纳米粒是一种可行的内皮抑素载体，用于肺癌的治疗。
The purpose of this study was to prepare ES-loaded chitosan nanoparticles (ES-NPs) and evaluate the antitumor effect of these particles on the Lewis lung cancer model. ES-NPs were prepared by a simple ionic cross-linking method. The characterization of the ES-NPs, including size distribution, zeta potential, loading efficiency and encapsulation efficiency (EE), was performed. An in vitro release test was also used to determine the release behavior of the ES-NPs. Cell viability and cell migration were assayed to detect the in vitro antiangiogenic effect of ES-NPs. In order to clarify the antitumor effect of ES-NPs in vivo, the Lewis lung cancer model was used. ES-NPs were successfully synthesized and shown to have a suitable size distribution and high EE. The nanoparticles were spherical and homogeneous in shape and exhibited an ideal releasing profile in vitro. Moreover, ES-NPs significantly inhibited the proliferation and migration of human umbilical vascular endothelial cells (HUVECs). The in vivo antiangiogenic activity was evaluated by ELISA and immunohistochemistry analyses, which revealed that ES-NPs had a stronger antiangiogenic effect for reinforced anticancer activity. Indeed, even the treatment cycle in which ES-NPs were injected every seven days, showed stronger antitumor effect than the free ES injected for 14 consecutive days. Our study confirmed that the CS nanoparticle is a feasible carrier for endostatin to be used in the treatment of lung cancer.
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影响因子：
12.4
作者：
Pawliuk, R;Bachelot, T;Leboulch, P
通讯作者：
Leboulch, P
影响因子：
--
作者：
Dong, Xiao-Peng;Xiao, Tian-Hui;Zhao, Xiao-Gang
通讯作者：
Zhao, Xiao-Gang
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影响因子：
8
作者：
Du Y;Zhang Q;Jing L;Liang X;Chi C;Li Y;Yang X;Dai Z;Tian J
通讯作者：
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影响因子：
3.1
作者：
Katas H;Raja MA;Lam KL
通讯作者：
Lam KL
影响因子：
14
作者：
Chen, Zhipeng;Zhang, Liujie;Li, Weidong
通讯作者：
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