LDL cholesterol performance of beta quantification reference measurement procedure.
LDL cholesterol performance of beta quantification reference measurement procedure.
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DOI：
10.1016/j.cca.2014.02.018
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发表时间：
2014-04-20
期刊：
影响因子：
--
通讯作者：
Miyamoto Y
中科院分区：
文献类型：
--
作者：
Nakamura M;Kayamori Y;Iso H;Kitamura A;Kiyama M;Koyama I;Nishimura K;Nakai M;Noda H;Dasti M;Vesper HW;Miyamoto Y
准确测量血脂在心血管疾病风险管理中至关重要。美国疾病控制和预防中心（CDC）胆固醇参考方法实验室网络（CRMLN）使用β定量（BQ）方法作为高密度脂蛋白胆固醇和低密度脂蛋白胆固醇（HDL-C，LDL-C）的参考测量程序（RMP），已确保这些测量的准确性超过20年。关于BQ RMP性能的数据有限。使用过去15年测量的280份血清样本，比较了CDC脂质参考实验室和日本CRMLN实验室超浓缩后的底部部分胆固醇（BFC）、HDL-C和LDL-C结果。采用方法比较和偏倚估计分析对数据进行统计学比较。BFC、HDL-C和LDL-C的CDC（x）和Osaka（y）之间的回归分析分别为y = 0.988x + 1.794（R2 = 0.997）、y = 0.980x + 1.118（R2 = 0.994）和y = 0.987x + 1.200（R2 = 0.997）。Osaka实验室符合CDC参考值的90%至95%的性能目标。大坂CRMLN实验室的BQ方法具有高度准确性，已稳定超过15年。准确测量BFC对于测定LDL-C至关重要。
Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements forover 20 years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP. Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15 years. Data were compared statistically using method comparison and bias estimation analysis. Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y = 0.988x + 1.794 (R2 = 0.997), y = 0.980x + 1.118 (R2 = 0.994), and y = 0.987x + 1.200 (R2 = 0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values. The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15 years. Accurate measurement of BFC is critical for the determination of LDL-C.
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影响因子：
4.4
作者：
Nakamura, Masakazu;Koyama, Isao;Ishikawa, Yoshinori
通讯作者：
Ishikawa, Yoshinori
影响因子：
1.8
作者：
Pisani, T;Pickering, S;Gebski, CP
通讯作者：
Gebski, CP
影响因子：
5.1
作者：
Imano, Hironori;Noda, Hiroyuki;Iso, Hiroyasu
通讯作者：
Iso, Hiroyasu
影响因子：
9.3
作者：
Miller WG;Myers GL;Sakurabayashi I;Bachmann LM;Caudill SP;Dziekonski A;Edwards S;Kimberly MM;Korzun WJ;Leary ET;Nakajima K;Nakamura M;Nilsson G;Shamburek RD;Vetrovec GW;Warnick GR;Remaley AT
通讯作者：
Remaley AT
影响因子：
4.4
作者：
Teramoto, Tamio;Sasaki, Jun;Yokode, Masayuki
通讯作者：
Yokode, Masayuki