Analysis of the effects of acoustic levitation to simulate the microgravity environment on the development of early zebrafish embryos.
Analysis of the effects of acoustic levitation to simulate the microgravity environment on the development of early zebrafish embryos.
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声悬浮模拟微重力环境对早期斑马鱼胚胎发育的影响分析
DOI：
10.1039/d0ra07344j
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发表时间：
2020-12-09
期刊：
影响因子：
3.9
通讯作者：
T V G K
中科院分区：
文献类型：
--
作者：
Li L;Gu N;Dong H;Li B;T V G K
在这项工作中，声学驻波场(ASWF)被用来模拟空间环境，这种环境具有微重力、无封闭和接触等特点。以斑马鱼胚胎为研究对象，作者在声场中饲养斑马鱼胚胎，以便研究在每个发育阶段和创造所使用的声悬浮(AL)技术的ASWF中对此类早期斑马鱼胚胎的生物学效应。通过这种方式，可以仔细地评估这些模拟空间环境的标本的生物安全性。对斑马鱼发育过程中的死亡率、畸形率、孵化率、自主活动和心率等重要指标进行了检测和分析。研究发现，在斑马鱼胚胎发育的早期阶段，ASWF对斑马鱼胚胎的发育有相当大的影响，在受精后0-8h(HPF)期间影响着卵裂、囊胚期和谷角期等特征。斑马鱼在使用所应用的AL处理后，表现出一些致畸特征，以及致死效应和孵化率显著下降。此外，观察到在这些条件下，自主活动和胚胎心率明显增加。然而，随着胚胎发育进入法氏囊咽部阶段(24-32hpf)，ASWF产生AL对斑马鱼的影响似乎几乎不显著，因为试验组和对照组的特征没有明显差异。所进行的实验为AL在这一领域的应用提供了科学参考，使这种斑马鱼胚胎在空间环境中发育的生物安全方面得以评估。
In this work, an acoustic standing wave field (ASWF) is used to simulate the space environment, which shows characteristics such as microgravity and the absence of containment and contact. Zebrafish embryos, used as the species under study in this work, were raised within the acoustic field by the authors, allowing the biological effects on such early zebrafish embryos, at each developmental stage and within the ASWF creating the acoustic levitation (AL) technology used, to be studied. In this way, the biological safety of thee specimens, simulating the space environment, could be carefully evaluated. Some important indexes of the process of zebrafish development, such as mortality, malformation rate, hatching rate, voluntary movement and heart rate were detected and analyzed. It has been found that the ASWF exerted considerable influence on the zebrafish embryos at the early development stage, influencing features such as the cleavage, blastula and gastrul stage, over the period 0–8 hour post fertilization (hpf). The zebrafish appear to show some features of teratogenesis, as well as lethal effects and a significant decrease of the hatching rate, after being treated by using the AL that was applied. Furthermore, it was observed that voluntary movements and the embryo heart rates apparently increased under these conditions. However, as the development of the embryo progressed into the bursa pharyngea stage (at 24–32 hpf), the influence of the ASWF creating the AL on zebrafish seemed almost to be insignificant, as there was no obvious difference between the characteristics of the experimental group and the control group. The experiment carried out has provided a scientific reference for the application of AL in this field, allowing the biological safety aspects of such zebrafish embryo development within a space environment to be evaluated.
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影响因子：
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作者：
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DOI：
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发表时间：
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