Quantitative glycoproteomics of human milk and association with atopic disease.
Quantitative glycoproteomics of human milk and association with atopic disease.
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DOI：
10.1371/journal.pone.0267967
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Jarvinen, Kirsi
中科院分区：
文献类型：
--
作者：
Holm, Matilda;Saraswat, Mayank;Joenvaara, Sakari;Seppo, Antti;Looney, R. John M.;Tohmola, Tiialotta;Renkonen, Jutta;Renkonen, Risto;Jarvinen, Kirsi
过敏性疾病和哮喘的流行在全球范围内迅速增加，环境和生活方式行为是其中的一个原因。流行病学研究表明，在农场长大的儿童患儿童特应性疾病的风险较低，这表明存在保护性的“农场效应”。纽约州北部的旧秩序门诺派(OOM)社区拥有传统的农业生活方式，特应性疾病的发生率很低，而且长期纯母乳喂养。人类乳蛋白是高度糖基化的，尽管对乳糖蛋白质组的研究很少。在这项研究中，我们使用定量糖蛋白组学比较了来自罗切斯特市区/郊区和OOM母亲的54个牛奶样本的N-糖蛋白谱，这两个群体具有不同的生活方式、暴露和特应性疾病的风险。我们还根据母亲和孩子是否患有特应性疾病对N-糖蛋白图谱进行了比较。我们从15种不同的蛋白质中鉴定了79个N-糖肽，发现包括免疫球蛋白A1、聚合免疫球蛋白受体和乳转铁蛋白在内的蛋白质具有显著的糖链异质性。我们发现，38个糖肽的丰度在罗切斯特和OOM母亲之间存在显著差异，并在所有比较中确定了四个丰度显著不同的糖肽。这四种糖肽可能与特应性疾病的发生有关。这项研究的结果表明，牛奶蛋白的差异糖基化可能与特应性疾病有关。
The prevalence of allergic diseases and asthma is increasing rapidly worldwide, with environmental and lifestyle behaviors implicated as a reason. Epidemiological studies have shown that children who grow up on farms are at lower risk of developing childhood atopic disease, indicating the presence of a protective “farm effect”. The Old Order Mennonite (OOM) community in Upstate New York have traditional, agrarian lifestyles, a low rate of atopic disease, and long periods of exclusive breastfeeding. Human milk proteins are heavily glycosylated, although there is a paucity of studies investigating the milk glycoproteome. In this study, we have used quantitative glycoproteomics to compare the N-glycoprotein profiles of 54 milk samples from Rochester urban/suburban and OOM mothers, two populations with different lifestyles, exposures, and risk of atopic disease. We also compared N-glycoprotein profiles according to the presence or absence of atopic disease in the mothers and, separately, the children. We identified 79 N-glycopeptides from 15 different proteins and found that proteins including immunoglobulin A1, polymeric immunoglobulin receptor, and lactotransferrin displayed significant glycan heterogeneity. We found that the abundances of 38 glycopeptides differed significantly between Rochester and OOM mothers and also identified four glycopeptides with significantly different abundances between all comparisons. These four glycopeptides may be associated with the development of atopic disease. The findings of this study suggest that the differential glycosylation of milk proteins could be linked to atopic disease.
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影响因子：
16.6
作者：
Steffen, Ulrike;Koeleman, Carolien A.;Schett, Georg
通讯作者：
Schett, Georg
影响因子：
12.4
作者：
通讯作者：
--
影响因子：
3.7
作者：
Joenvaara S;Saraswat M;Kuusela P;Saraswat S;Agarwal R;Kaartinen J;Järvinen A;Renkonen R
通讯作者：
Renkonen R
DOI：
10.1186/1939-4551-7-12
发表时间：
2014
期刊：
The World Allergy Organization journal
影响因子：
--
作者：
Pawankar R
通讯作者：
Pawankar R
影响因子：
3.7
作者：
Hettinga, Kasper A.;Reina, Fabiola M.;Wijga, Alet H.
通讯作者：
Wijga, Alet H.