Imaging in vivo secondary caries and ex vivo dental biofilms using cross-polarization optical coherence tomography.
Imaging in vivo secondary caries and ex vivo dental biofilms using cross-polarization optical coherence tomography.
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使用交叉极化光学相干断层扫描中的体内次要龋齿和离体牙科生物膜进行成像。
DOI：
10.1016/j.dental.2012.04.004
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发表时间：
2012-07
期刊：
影响因子：
5
通讯作者：
Jones, Robert S.
中科院分区：
文献类型：
--
作者：
Lenton, Pat;Rudney, Joel;Chen, Ruoqiong;Fok, Alex;Aparicio, Conrado;Jones, Robert S.
关键词：
传统的诊断方法往往只能检测到晚期釉质脱矿的复合树脂漂白。本研究的目的是使用一种新型的光学相干断层扫描系统与口内探头检查亚表面牙齿复合界面，并评估儿童患者继发龋的存在。采用一种新设计的口内交叉偏振扫描光源光学相干断层成像系统（CP-OCT），对牙釉质-复合材料界面的完整性进行了活体检测。招募了22名儿童受试者，他们的乳牙中有最近放置的或长期放置的复合材料牙弓。为了更好地理解细菌生物膜如何引起界面处的脱矿，我们还使用口内CP-OCT系统来评估牙科复合材料上的离体细菌生物膜生长。作为阳性对照，与最近放置的复合牙釉质相关的界面相比，空化的继发性龋坏界面显示出18.2dB的增加（p<0.001）或超过1-2个数量级的散射。几个长期存在的复合材料涂层，临床上看起来是合理的，比最近放置的涂层散射明显增加。这表明CP-OCT能够评估界面降解，例如空化之前的早期继发性龋齿。CP-OCT还能够对牙科复合材料上的离体生物膜进行成像并评估其厚度。本文表明，使用基于分束器的设计的CP-OCT成像可以检查人类受试者的牙科复合材料的表面下界面。此外，CP-OCT系统的探头尺寸和采集速度允许分析儿童的龋齿发展。
Conventional diagnostic methods frequently detect only late stage enamel demineralization under composite resin restorations. The objective of this study is to examine the subsurface tooth-composite interface and to assess for the presence of secondary caries in pediatric patients using a novel Optical Coherence Tomography System with an intraoral probe. A newly designed intraoral cross polarization swept source optical coherence tomography (CP-OCT) imaging system was used to examine the integrity of the enamel-composite interfaces in vivo. Twenty two pediatric subjects were recruited with either recently placed or long standing composite restorations in their primary teeth. To better understand how bacterial biofilms cause demineralization at the interface, we also used the intraoral CP-OCT system to assess ex vivo bacterial biofilm growth on dental composites. As a positive control, cavitated secondary carious interfaces showed a 18.2 dB increase (p<0.001), or over 1-2 orders of magnitude higher, scattering than interfaces associated with recently placed composite restorations. Several long standing composite restorations, which appeared clinically sound, had a marked increase in scattering than recently placed restorations. This suggests the ability of CP-OCT to assess interfacial degradation such as early secondary caries prior to cavitation. CP-OCT was also able to image ex vivo biofilms on dental composites and assess their thickness. This paper shows that CP-OCT imaging using a beam splitter based design can examine the subsurface interface of dental composites in human subjects. Furthermore, the probe dimensions and acquisition speed of the CP-OCT system allowed for analysis of caries development in children.
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影响因子：
3.8
作者：
Jones, RS;Huynh, GD;Fried, D
通讯作者：
Fried, D
影响因子：
3.6
作者：
Golubovic, B;Bouma, BE;Fujimoto, JG
通讯作者：
Fujimoto, JG
影响因子：
3.5
作者：
Liu, Bin;Brezinski, Mark E.
通讯作者：
Brezinski, Mark E.
DOI：
10.1016/s1359-835x(98)00084-0
发表时间：
1999-01-01
影响因子：
8.7
作者：
Dunkers, JP;Parnas, RS;Bouma, BE
通讯作者：
Bouma, BE
影响因子：
3.5
作者：
Ko, ACT;Choo-Smith, LP;Cleghorn, B
通讯作者：
Cleghorn, B