Analysis of the developmental stages, kinetics, and phenotypes exhibited by myeloid cells driven by GM-CSF in vitro.
Analysis of the developmental stages, kinetics, and phenotypes exhibited by myeloid cells driven by GM-CSF in vitro.
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体外 GM-CSF 驱动的骨髓细胞的发育阶段、动力学和表型分析。
DOI：
10.1371/journal.pone.0181985
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Hiltbold Schwartz E
中科院分区：
文献类型：
--
作者：
Rogers PB;Driessnack MG;Hiltbold Schwartz E
常规DC的发育进程已经相当明确，但单核细胞衍生的GM-CSF驱动的DC的发育途径还不太清楚。我们通过建立鉴定五种不同GM-CSF衍生细胞类型的分离策略来解决这个问题。Ly 6C和CD 115（Csf-1 R）的表达被用来鉴定和分离四个群体。其中一个群体可以进一步分离的基础上CD 11 c的表达，区分五个群体。我们进一步定义了这些细胞的基础上表达的转录因子和标记物的早期和晚期阶段的骨髓发展。这些离散的发育阶段与先前定义的群体很好地对应：共同髓样祖细胞（CMP）、粒细胞/巨噬细胞祖细胞（GMP）、单核细胞以及单核细胞衍生的巨噬细胞（moMac）和单核细胞衍生的DC（moDC）。最后，在moMac群体中，我们还鉴定了moDC前体活性（moDP），根据其MHC II类表达水平和发育可塑性，可以将其与moMac和moDC区分开来。
The developmental progression of conventional DC has been quite well defined, yet the developmental pathway of monocyte-derived, GM-CSF-driven DC is less well understood. We addressed this issue by establishing an isolation strategy that identifies five distinct GM-CSF derived cell types. Expression of Ly6C and CD115 (Csf-1R) was used to identify and isolate four populations. One of the populations could be further separated based on CD11c expression, distinguishing five populations. We further defined these cells based on expression of transcription factors and markers of early and later stages of myeloid development. These discreet developmental stages corresponded well with previously defined populations: Common Myeloid Progenitors (CMP), Granulocyte/Macrophage Progenitors (GMP), Monocytes, as well as Monocyte-derived macrophages (moMac) and Monocyte-derived DC (moDC). Finally, within the moMac population we also identified moDC precursor activity (moDP) that could be distinguished from moMac and moDC based on their level of MHC class II expression and developmental plasticity.
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影响因子：
30.5
作者：
Naik, Shalin H.;Sathe, Priyanka;Shortman, Ken
通讯作者：
Shortman, Ken
影响因子：
11.4
作者：
Feinberg, Mark W.;Wara, Akm Khyrul;Jain, Mukesh K.
通讯作者：
Jain, Mukesh K.
影响因子：
15.3
作者：
King, Irah L.;Kroenke, Mark A.;Segal, Benjamin M.
通讯作者：
Segal, Benjamin M.
影响因子：
0.9
作者：
Katayama, H;Hattori, Y;Kimura, H
通讯作者：
Kimura, H
DOI：
10.4049/jimmunol.1002587
发表时间：
2011-02-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Atibalentja DF;Murphy KM;Unanue ER
通讯作者：
Unanue ER