A 19-nucleotide insertion in the leader sequence of avian leukosis virus subgroup J contributes to its replication in vitro but is not related to its pathogenicity in vivo.
A 19-nucleotide insertion in the leader sequence of avian leukosis virus subgroup J contributes to its replication in vitro but is not related to its pathogenicity in vivo.
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禽白血病病毒 J 亚群前导序列中的 19 个核苷酸插入有助于其体外复制，但与其体内致病性无关
DOI：
10.1371/journal.pone.0084797
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Ji X;Wang Q;Li X;Qi X;Wang Y;Gao H;Gao Y;Wang X
禽白血病病毒J亚群（ALV-J）首次从发生髓性白血病的肉鸡中分离出来，自2008年以来，ALV-J感染在中国鸡群中变得普遍。将ALV-J流行分离株与ALV-J原型病毒hrs -103的序列进行比较，发现了几个明显的特征，其中一个特征是在先导序列中插入了19个核苷酸（nt）。为了确定19-nt插入在ALV-J致病性中的作用，我们构建并挽救了一对病毒。第一个病毒是ALV-J中国分离株（编号为rSD1009），其先导序列中含有19-nt插入。第二种病毒是克隆病毒，其先导序列有一个缺失的19-nt序列（编号为rSD1009△19）。与rSD1009△19相比，rSD1009在体外表现出中等的生长优势。然而，两种获救病毒在鸡体内的病毒复制和致癌性均无差异。这些结果表明，19-nt的插入促进了ALV-J在体外的复制，但与体内的致病性无关。
Subgroup J avian leukosis virus (ALV-J) was first isolated from meat-type chickens that had developed myeloid leukosis and since 2008, ALV-J infections in chickens have become widespread in China. A comparison of the sequence of ALV-J epidemic isolates with HPRS-103, the ALV-J prototype virus, revealed several distinct features, one of which is a 19-nucleotide (nt) insertion in the leader sequence. To determine the role of the 19-nt insertion in ALV-J pathogenicity, a pair of viruses were constructed and rescued. The first virus was an ALV-J Chinese isolate (designated rSD1009) containing the 19-nt insertion in its leader sequence. The second virus was a clone, in which the leader sequence had a deleted 19-nt sequence (designated rSD1009△19). Compared with rSD1009△19, rSD1009 displayed a moderate growth advantage in vitro. However, no differences were demonstrated in either viral replication or oncogenicity between the two rescued viruses in chickens. These results indicated that the 19-nt insertion contributed to ALV-J replication in vitro but was not related to its pathogenicity in vivo.
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