Expression of amphiphysin I in Sertoli cells and its implication in spermatogenesis.
Expression of amphiphysin I in Sertoli cells and its implication in spermatogenesis.
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支持细胞中两性蛋白 I 的表达及其在精子发生中的意义。
DOI：
10.1006/bbrc.2001.5650
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发表时间：
2001
影响因子：
3.1
通讯作者：
A. Tokunaga
中科院分区：
文献类型：
--
作者：
M. Watanabe;K. Tsutsui;O. Hosoya;H. Kumon;A. Tokunaga
Amphiphysin I 是一种集中在神经末梢的蛋白质，参与突触小泡膜的内吞作用。我们在此表明，两性蛋白 I 在大鼠睾丸中表达，仅位于支持细胞中。在出生后睾丸发育中，两性蛋白 I 的表达在出生时并不明显，但在出生后第 15 天（P15）变得显着，与精子发生的开始一致。两性蛋白I的表达水平在P15和P25之间增加10倍，达到成年水平。在因施用乙烷二甲磺酸盐而造成可逆性损伤的成年睾丸中，两性蛋白 I 的表达在损伤后没有改变，而该蛋白质短暂地转化为其磷酸化形式。磷酸化两性蛋白 I 水平的增加与生殖细胞的严重组织学损伤密切相关。目前的研究结果表明，支持细胞中的两性蛋白 I 可能通过内吞过程参与精子发生。
Amphiphysin I is a protein concentrated in nerve terminals and involved in the endocytosis of synaptic vesicle membrane. We show here that amphiphysin I is expressed in the rat testis, localized exclusively in the Sertoli cells. In the postnatal testicular development, expression of amphiphysin I was not evident at birth, but became significant at postnatal day 15 (P15), coinciding with the onset of spermatogenesis. The expression level of amphiphysin I increased 10-fold between P15 and P25 to reach the adult level. In adult testes reversibly damaged by ethane dimethane sulphonate administration, expression of amphiphysin I did not change following the damage, whereas the protein was transiently converted into its phosphorylated form. The increase in levels of phosphorylated amphiphysin I was closely associated with the severe histological damage to germ cells. The present findings suggest that amphiphysin I in Sertoli cells is involved in spermatogenesis, probably through endocytic processes.
影响因子：
56.9
作者：
Slepnev, VI;Ochoa, GC;De Camilli, P
通讯作者：
De Camilli, P
影响因子：
--
作者：
RUSSELL, LD;BARTKE, A;GOH, JC
通讯作者：
GOH, JC
DOI：
10.1073/pnas.93.1.331
发表时间：
1996-01-09
影响因子：
11.1
作者：
David, C;McPherson, PS;DeCamilli, P
通讯作者：
DeCamilli, P