Transforming growth factor Beta 1 stimulates profibrotic activities of luteal fibroblasts in cows.
Transforming growth factor Beta 1 stimulates profibrotic activities of luteal fibroblasts in cows.
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DOI：
10.1095/biolreprod.112.100735
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发表时间：
2012-11
影响因子：
3.6
通讯作者：
Davis JS
中科院分区：
文献类型：
--
作者：
Maroni D;Davis JS
黄体溶解的特征是血管退化、黄体细胞凋亡和以胶原沉积为特征的细胞外基质重塑1。转化生长因子- 1 （TGFB1）通过刺激细胞外基质成分的合成，是伤口愈合和纤维化过程的有效介质。我们假设TGFB1刺激黄体成纤维细胞的促纤维化活性。我们检测了TGFB1对黄体成纤维细胞增殖、细胞外基质生成、漂浮凝胶收缩和趋化性的作用。从牛黄体中分离成纤维细胞。Western blot分析显示，黄体成纤维细胞表达胶原1和脯氨酰4-羟化酶，但不表达内皮细胞或类固醇细胞的标志物。TGFB1治疗成纤维细胞可刺激SMAD2和SMAD3的磷酸化。[3H]胸腺嘧啶掺入研究表明，TGFB1引起黄体成纤维细胞DNA合成的浓度依赖性降低，并显著（P < 0.05）降低FGF2和胎牛血清的增殖作用。然而，TGFB1并没有降低黄体成纤维细胞的活力。用TGFB1处理黄体成纤维细胞诱导层粘连蛋白、胶原蛋白1和基质金属蛋白酶1的表达，通过Western blot分析和条件培养基明胶酶谱法检测。TGFB1在transwell系统中增加了黄体成纤维细胞对纤维连接蛋白的趋化性。此外，TGFB1增加了成纤维细胞介导的漂浮牛胶原1凝胶的收缩。这些结果表明，TGFB1通过刺激黄体成纤维细胞重塑和收缩细胞外基质，促进黄体结构退化。
Luteolysis is characterized by angioregression, luteal cell apoptosis, and remodeling of the extracellular matrix characterized by deposition of collagen 1. Transforming growth factor beta 1 (TGFB1) is a potent mediator of wound healing and fibrotic processes through stimulation of the synthesis of extracellular matrix components. We hypothesized that TGFB1 stimulates profibrotic activities of luteal fibroblasts. We examined the actions of TGFB1 on luteal fibroblast proliferation, extracellular matrix production, floating gel contraction, and chemotaxis. Fibroblasts were isolated from the bovine corpus luteum. Western blot analysis showed that luteal fibroblasts expressed collagen 1 and prolyl 4-hydroxylase but did not express markers of endothelial or steroidogenic cells. Treatment of fibroblasts with TGFB1 stimulated the phosphorylation of SMAD2 and SMAD3. [3H]thymidine incorporation studies showed that TGFB1 caused concentration-dependent reductions in DNA synthesis in luteal fibroblasts and significantly (P < 0.05) reduced the proliferative effect of FGF2 and fetal calf serum. However, TGFB1 did not reduce the viability of luteal fibroblasts. Treatment of luteal fibroblasts with TGFB1 induced the expression of laminin, collagen 1, and matrix metalloproteinase 1 as determined by Western blot analysis and gelatin zymography of conditioned medium. TGFB1 increased the chemotaxis of luteal fibroblasts toward fibronectin in a transwell system. Furthermore, TGFB1 increased the fibroblast-mediated contraction of floating bovine collagen 1 gels. These results suggest that TGFB1 contributes to the structural regression of the corpus luteum by stimulating luteal fibroblasts to remodel and contract the extracellular matrix.
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影响因子：
20.1
作者：
Dobaczewski M;Bujak M;Li N;Gonzalez-Quesada C;Mendoza LH;Wang XF;Frangogiannis NG
通讯作者：
Frangogiannis NG
影响因子：
3.6
作者：
Friedman, A;Weiss, S;Meidan, R
通讯作者：
Meidan, R
影响因子：
2.9
作者：
Pratsinis, H;Giannouli, CC;Kletsas, D
通讯作者：
Kletsas, D
DOI：
10.1530/ror.0.0050012
发表时间：
2000-01-01
期刊：
REVIEWS OF REPRODUCTION
影响因子：
--
作者：
Duncan, WC
通讯作者：
Duncan, WC
影响因子：
1.4
作者：
Grinnell, Frederick
通讯作者：
Grinnell, Frederick