Restoration of normal growth control and membrane antigen composition in malignant cells by N,N-dimethylformamide.
Restoration of normal growth control and membrane antigen composition in malignant cells by N,N-dimethylformamide.
复制标题
N,N-二甲基甲酰胺恢复恶性细胞的正常生长控制和膜抗原组成。
作者：
S. Chakrabarty;L. J. Mcrae;A. Levine;M. Brattain
研究了分化剂N，N-二甲基甲酰胺（DMF）对恶性AKR-MCA细胞的作用。将DMF处理的AKR-MCA细胞的性质与正常亲本AKR-2B小鼠胚胎成纤维细胞的性质进行比较。通过几个标准，发现在1%DMF中生长的AKR-MCA细胞与其正常对应物比未处理的AKR-MCA细胞更相似。这些标准包括转化形态的丧失、倍增时间减少2倍、饱和密度减少10倍以及完全丧失以锚定独立性生长的能力。发现与正常AKR-2B细胞相比，AKR-MCA细胞中的高分子量膜抗原（Mr 110，000至450，000）的表达大大降低，但通过用DMF处理AKR-MCA细胞恢复了高分子量膜抗原的表达。另一方面，发现低分子量AKR-MCA细胞相关膜抗原的表达被抑制。对这些细胞的促有丝分裂反应的研究表明，AKR-MCA和AKR-2B细胞可能受到不同类型的生长控制的调节。生长停滞的AKR-MCA细胞对表皮生长因子没有反应，但对营养补充有反应。另一方面，AKR-2B细胞对表皮生长因子有反应，但对营养补充没有反应。用DMF处理AKR-MCA细胞恢复了其对表皮生长因子的反应能力，而其对营养补充的反应能力丧失。本研究的结果表明，DMF处理诱导了恶性AKR-MCA细胞在膜抗原组成和生长控制特性方面的正常化。
The effects of the differentiation agent, N,N-dimethylformamide (DMF), on malignant AKR-MCA cells were studied. The properties of DMF-treated AKR-MCA cells were compared to those of the normal parental AKR-2B mouse embryo fibroblasts. AKR-MCA cells grown in 1% DMF were found to be more similar to their normal counterparts than to untreated AKR-MCA cells by several criteria. These criteria included the loss of the transformed morphology, a 2-fold reduction of doubling time, a 10-fold reduction of saturation density, and the complete loss of the ability to grow with anchorage independence. The expression of high-molecular-weight membrane antigens (Mr 110,000 to 450,000), which was found to be greatly reduced in AKR-MCA cells in comparison to normal AKR-2B cells, was restored by treatment of AKR-MCA cells with DMF. The expression of a low-molecular-weight AKR-MCA cell-associated membrane antigen, on the other hand was found to be suppressed. Studies on the mitogenic response of these cells indicated that AKR-MCA and AKR-2B cells may be regulated by different types of growth control. Growth-arrested AKR-MCA cells did not respond to epidermal growth factor, but responded to nutrient replenishment. AKR-2B cells, on the other hand, responded to epidermal growth factor, but did not respond to nutrient replenishment. Treatment of AKR-MCA cells with DMF restored their ability to respond to epidermal growth factor, while their ability to respond to nutrient replenishment was lost. The results of this study indicated that DMF treatment induced the normalization of malignant AKR-MCA cells with regard to membrane antigen composition and growth control properties.
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影响因子：
56.9
作者：
DOOLITTLE, RF;HUNKAPILLER, MW;ANTONIADES, HN
通讯作者：
ANTONIADES, HN
极性溶剂抑制人结肠癌异种移植物的生长。
影响因子：
11.2
作者：
Dexter,DL;Spremulli,EN;Matook,GM;Diamond,I;Calabresi,P
通讯作者：
Calabresi,P
Novikoff 肝癌抗原蛋白 p39、p49 和 p56 的组织特异性和细胞分布。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Schmidt,WN;Stryjecka-Zimmer,M;Glass2nd,WF;Briggs,RC;Hnilica,LS
通讯作者：
Hnilica,LS
鉴定从聚丙烯酰胺凝胶转移至硝化纤维的组织特异性核抗原。
DOI：
10.1126/science.7003713
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Glass2nd,WF;Briggs,RC;Hnilica,LS
通讯作者：
Hnilica,LS
影响因子：
3.8
作者：
Colleran, Gabrielle;McInerney, Niall;Sawyer, Elinor
通讯作者：
Sawyer, Elinor