Topical Treatment with Xiaozheng Zhitong Paste (XZP) Alleviates Bone Destruction and Bone Cancer Pain in a Rat Model of Prostate Cancer-Induced Bone Pain by Modulating the RANKL/RANK/OPG Signaling.
Topical Treatment with Xiaozheng Zhitong Paste (XZP) Alleviates Bone Destruction and Bone Cancer Pain in a Rat Model of Prostate Cancer-Induced Bone Pain by Modulating the RANKL/RANK/OPG Signaling.
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消症止痛膏 (XZP) 局部治疗通过调节 RANKL/RANK/OPG 信号传导减轻前列腺癌引起的骨痛大鼠模型中的骨破坏和骨癌疼痛。
DOI：
10.1155/2015/215892
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Hua B
中科院分区：
文献类型：
--
作者：
Bao Y;Gao Y;Du M;Hou W;Yang L;Kong X;Zheng H;Li W;Hua B
为探讨消正止痛膏（XZP）对骨癌疼痛的治疗作用及其机制，采用Wistar大鼠胫骨接种载体或前列腺癌PC-3， XZP剂量分别为15.75、31.5、63 g/kg，每天2次，连续治疗21 d。检测小鼠骨结构损伤、痛觉行为、破骨细胞和成骨细胞活性、OPG、RANL、RNAK、PTHrP、IGF-1、M-CSF、IL-8、TNF-α水平。与安慰剂组相比，xzp治疗大鼠的侵袭癌细胞数量明显减少，骨损伤水平、机械阈值水平和足爪退断潜伏期明显降低，血清TRACP5b、ICTP、PINP和BAP水平明显降低，成骨细胞和破骨细胞活性水平明显降低（P<0.05）。xzp处理大鼠骨OPG水平显著升高，RANL、RNAK、PTHrP、IGF-1、M-CSF、IL-8、TNF-α水平显著降低（P<0.05）。XZP通过调节RANKL/RANK/OPG通路和骨癌相关炎症，显著减轻大鼠癌性骨损伤和骨破骨细胞和成骨细胞活性，减轻前列腺癌性骨痛。
To explore the effects and mechanisms of Xiaozheng Zhitong Paste (XZP) on bone cancer pain, Wistar rats were inoculated with vehicle or prostate cancer PC-3 into the tibia bone and treated topically with inert paste, XZP at 15.75, 31.5, or 63 g/kg twice per day for 21 days. Their bone structural damage, nociceptive behaviors, bone osteoclast and osteoblast activity, and the levels of OPG, RANL, RNAK, PTHrP, IGF-1, M-CSF, IL-8, and TNF-α were examined. In comparison with that in the placebo group, significantly reduced numbers of invaded cancer cells, decreased levels of bone damage and mechanical threshold and paw withdrawal latency, lower levels of serum TRACP5b, ICTP, PINP, and BAP, and less levels of bone osteoblast and osteoclast activity were detected in the XZP-treated rats (P<0.05). Moreover, significantly increased levels of bone OPG but significantly decreased levels of RANL, RNAK, PTHrP, IGF-1, M-CSF, IL-8, and TNF-α were detected in the XZP-treated rats (P<0.05 for all). Together, XZP treatment significantly mitigated the cancer-induced bone damage and bone osteoclast and osteoblast activity and alleviated prostate cancer-induced bone pain by modulating the RANKL/RANK/OPG pathway and bone cancer-related inflammation in rats.
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