Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats.
Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats.
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益生素低聚木糖改善高脂饮食诱导的大鼠肝脂肪变性
DOI：
10.3390/nu12113225
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发表时间：
2020-10-22
期刊：
影响因子：
5.9
通讯作者：
Pekkala S
中科院分区：
文献类型：
--
作者：
Lensu S;Pariyani R;Mäkinen E;Yang B;Saleem W;Munukka E;Lehti M;Driuchina A;Lindén J;Tiirola M;Lahti L;Pekkala S
了解肠道微生物群（GM）在非酒精性脂肪肝（NAFLD）中的重要性，为治疗微生物带来了希望。我们已经表明，高肝脂肪含量与人体中普氏粪杆菌的低丰度相关，进一步，给予普氏粪杆菌。prausnitzii预防小鼠NAFLD。在这里，我们瞄准F。通过益生元低聚木糖（XOS）治疗NAFLD。第一，XOS对F.在体外评估普氏菌的生长。然后，Wistar大鼠（n = 10/组）补充或不补充XOS高（HFD，60%的能量来自脂肪）或低（LFD）脂肪饮食12周。XOS提高了F. prausnitzii的增长，只有轻微的影响，对转基因成分。当补充HFD时，XOS改善肝脂肪变性。其潜在机制涉及肝脏β-氧化和线粒体呼吸增强。盲肠代谢物的核磁共振（1H-NMR）分析显示，与HFD相比，LFD组具有更健康的盲肠短链脂肪酸谱，并且在HFD上，XOS减少了盲肠异戊酸和酪氨酸，这些代谢物先前与NAFLD相关。盲肠支链脂肪酸与肝甘油三酯呈正相关，丁酸与肝甘油三酯呈负相关。总之，补充低聚木糖可通过改善肝脏氧化代谢和影响GM来改善NAFLD。
Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes. We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice. Here, we aimed at targeting F. prausnitzii by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD. First, the effect of XOS on F. prausnitzii growth was assessed in vitro. Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group). XOS increased F. prausnitzii growth, having only a minor impact on the GM composition. When supplemented with HFD, XOS ameliorated hepatic steatosis. The underlying mechanisms involved enhanced hepatic β-oxidation and mitochondrial respiration. Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD. Cecal branched-chain fatty acids associated positively and butyrate negatively with hepatic triglycerides. In conclusion, XOS supplementation can ameliorate NAFLD by improving hepatic oxidative metabolism and affecting GM.
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影响因子：
4.6
作者：
Gulhane M;Murray L;Lourie R;Tong H;Sheng YH;Wang R;Kang A;Schreiber V;Wong KY;Magor G;Denman S;Begun J;Florin TH;Perkins A;Cuív PÓ;McGuckin MA;Hasnain SZ
通讯作者：
Hasnain SZ
影响因子：
4.2
作者：
Mariat D;Firmesse O;Levenez F;Guimarăes V;Sokol H;Doré J;Corthier G;Furet JP
通讯作者：
Furet JP
影响因子：
13.5
作者：
Gaggini, Melania;Carli, Fabrizia;Gastaldelli, Amalia
通讯作者：
Gastaldelli, Amalia
影响因子：
11
作者：
Munukka, Eveliina;Rintala, Anniina;Pekkala, Satu
通讯作者：
Pekkala, Satu
影响因子：
13.5
作者：
Mouzaki, Marialena;Comelli, Elena M.;Allard, Johane P.
通讯作者：
Allard, Johane P.