Surface functionalization of polyurethane scaffolds mimicking the myocardial microenvironment to support cardiac primitive cells.
Surface functionalization of polyurethane scaffolds mimicking the myocardial microenvironment to support cardiac primitive cells.
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DOI：
10.1371/journal.pone.0199896
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Chiono V
中科院分区：
文献类型：
--
作者：
Boffito M;Di Meglio F;Mozetic P;Giannitelli SM;Carmagnola I;Castaldo C;Nurzynska D;Sacco AM;Miraglia R;Montagnani S;Vitale N;Brancaccio M;Tarone G;Basoli F;Rainer A;Trombetta M;Ciardelli G;Chiono V
用人心脏祖细胞（CPC）填充的支架代表了心肌梗死后心脏再生的治疗机会。在这项工作中，正方形网格支架是由聚氨酯（PU）通过熔融挤出增材制造，进一步进行等离子体处理丙烯酸表面接枝/聚合，并最终接枝层粘连蛋白-1（PU-LN 1）或明胶（PU-G）通过碳二亚胺化学。LN 1是一种心脏小生境细胞外基质成分，在胚胎发育期间的心脏形成中起关键作用，而G是一种低成本的细胞粘附蛋白，在此用作控制功能化分子。X射线光电子能谱分析表明，官能化后氮百分比增加。O 1 s和C1 s核心水平的光谱和静态接触角的测量结果表明与成功的功能化的变化。ELISA测定证实了LN 1表面接枝。PU-G和PU-LN 1支架均能改善CPC粘附，但LN 1功能化在促进增殖、保护免于凋亡以及心肌细胞、内皮细胞和平滑肌细胞的分化标志物表达方面更上级。PU-LN 1和PU支架被生物降解为非细胞毒性残留物。皮下植入小鼠的支架引起弱炎症并与宿主组织整合，证明支架周围有显著的血管密度。PU-LN 1支架在驱动CPC行为方面显示出其优越性，证明其在心肌再生医学中的前景。
Scaffolds populated with human cardiac progenitor cells (CPCs) represent a therapeutic opportunity for heart regeneration after myocardial infarction. In this work, square-grid scaffolds are prepared by melt-extrusion additive manufacturing from a polyurethane (PU), further subjected to plasma treatment for acrylic acid surface grafting/polymerization and finally grafted with laminin-1 (PU-LN1) or gelatin (PU-G) by carbodiimide chemistry. LN1 is a cardiac niche extracellular matrix component and plays a key role in heart formation during embryogenesis, while G is a low-cost cell-adhesion protein, here used as a control functionalizing molecule. X-ray photoelectron spectroscopy analysis shows nitrogen percentage increase after functionalization. O1s and C1s core-level spectra and static contact angle measurements show changes associated with successful functionalization. ELISA assay confirms LN1 surface grafting. PU-G and PU-LN1 scaffolds both improve CPC adhesion, but LN1 functionalization is superior in promoting proliferation, protection from apoptosis and expression of differentiation markers for cardiomyocytes, endothelial and smooth muscle cells. PU-LN1 and PU scaffolds are biodegraded into non-cytotoxic residues. Scaffolds subcutaneously implanted in mice evoke weak inflammation and integrate with the host tissue, evidencing a significant blood vessel density around the scaffolds. PU-LN1 scaffolds show their superiority in driving CPC behavior, evidencing their promising role in myocardial regenerative medicine.
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抑制层粘连蛋白α1-链表达会导致基底膜组装和细胞分化的改变。
影响因子：
7.8
作者：
De Arcangelis, A;Neuville, P;Boukamel, R;Lefebvre, O;Kedinger, M;Simon-Assmann, P
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影响因子：
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DOI：
10.1016/j.msec.2015.03.002
发表时间：
2015-06-01
影响因子：
7.9
作者：
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通讯作者：
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影响因子：
5.2
作者：
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