Temperature profiles of different cooling methods in porcine pancreas procurement.
Temperature profiles of different cooling methods in porcine pancreas procurement.
复制标题
猪胰腺采购中不同冷却方法的温度曲线。
DOI：
10.1111/xen.12114
复制
发表时间：
2014-11
影响因子：
3.9
通讯作者：
Papas KK
中科院分区：
文献类型：
--
作者：
Weegman BP;Suszynski TM;Scott WE 3rd;Ferrer Fábrega J;Avgoustiniatos ES;Anazawa T;O'Brien TD;Rizzari MD;Karatzas T;Jie T;Sutherland DE;Hering BJ;Papas KK
Porcine islet xenotransplantation is a promising alternative to human islet allotransplantation. Porcine pancreas cooling needs to be optimized to reduce the warm ischemia time (WIT) following donation after cardiac death, which is associated with poorer islet isolation outcomes. This study examines the effect of 4 different cooling Methods on core porcine pancreas temperature (n=24) and histopathology (n=16). All Methods involved surface cooling with crushed ice and chilled irrigation. Method A, which is the standard for porcine pancreas procurement, used only surface cooling. Method B involved an intravascular flush with cold solution through the pancreas arterial system. Method C involved an intraductal infusion with cold solution through the major pancreatic duct, and Method D combined all 3 cooling Methods. Surface cooling alone (Method A) gradually decreased core pancreas temperature to < 10 °C after 30 minutes. Using an intravascular flush (Method B) improved cooling during the entire duration of procurement, but incorporating an intraductal infusion (Method C) rapidly reduced core temperature 15–20 °C within the first 2 minutes of cooling. Combining all methods (Method D) was the most effective at rapidly reducing temperature and providing sustained cooling throughout the duration of procurement, although the recorded WIT was not different between Methods (p=0.36). Histological scores were different between the cooling Methods (p=0.02) and the worst with Method A. There were differences in histological scores between Methods A and C (p=0.02) and Methods A and D (p=0.02), but not between Methods C and D (p=0.95), which may highlight the importance of early cooling using an intraductal infusion. In conclusion, surface cooling alone cannot rapidly cool large (porcine or human) pancreata. Additional cooling with an intravascular flush and intraductal infusion results in improved core porcine pancreas temperature profiles during procurement and histopathology scores. These data may also have implications on human pancreas procurement since use of an intraductal infusion is not common practice.
登录
查看更多内容
影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
T细胞介导的免疫障碍物的异种移植。
影响因子：
3.9
作者：
Scalea J;Hanecamp I;Robson SC;Yamada K
通讯作者：
Yamada K
DOI：
10.1111/j.1600-6143.2010.03027.x
发表时间：
2010-04
期刊：
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
影响因子：
--
作者：
Zhao G;Moore DJ;Lee KM;Kim JI;Duff PE;O'Connor MR;Hirohashi T;Lei J;Yang M;Markmann JF;Deng S
通讯作者：
Deng S
影响因子：
4.4
作者：
Deng, Shaoping;Moore, Daniel J.;Markmann, James F.
通讯作者：
Markmann, James F.
影响因子：
6.4
作者：
Triponez, F;Oberholzer, J;Lou, J
通讯作者：
Lou, J