PEG Reinforced Scaffold Promotes Uniform Distribution of Human MSC-Created Cartilage Matrix.
PEG Reinforced Scaffold Promotes Uniform Distribution of Human MSC-Created Cartilage Matrix.
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DOI：
10.3390/gels8120794
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发表时间：
2022-12-03
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影响因子：
--
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之前，我们使用明胶/透明质酸（GH）为基础的支架来诱导人骨髓源间充质基质细胞（hBMSC）的软骨分化。结果表明，hBMSCs具有强大的软骨形成能力，促进了体内软骨再生。然而，我们注意到生长激素支架的压缩模量明显低于天然软骨。在这项研究中，我们旨在提高生长激素支架的机械强度，同时不显著损害其软骨支持性能。具体来说，聚乙二醇二丙烯酸酯（PEGDA）和光引发剂渗透到预先形成的负载hbmsc的GH支架中，然后光交联。结果表明，在软骨形成初期，PEG的浸润显著增加了支架中心区域的糖胺聚糖（GAGs）的沉积。为了探讨其机制，我们比较了GH和PEG浸润的GH+PEG支架（GH+PEG）边缘和中心区域的细胞迁移和增殖情况。两组均观察到有限的细胞迁移，但GH组比GH+PEG组观察到更多的增殖细胞。最后，用牛软骨组织进行体外修复研究表明，PEG-浸渍支架与宿主组织结合良好。这些结果表明，将PEG渗透到预成型的GH支架中制备的PEG-GH杂交支架具有良好的整合能力，是修复软骨损伤的新工具。
Previously, we used a gelatin/hyaluronic acid (GH)-based scaffold to induce chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells (hBMSC). The results showed that hBMSCs underwent robust chondrogenesis and facilitated in vivo cartilage regeneration. However, it was noticed that the GH scaffolds display a compressive modulus that is markedly lower than native cartilage. In this study, we aimed to enhance the mechanical strength of GH scaffolds without significantly impairing their chondrosupportive property. Specifically, polyethylene glycol diacrylate (PEGDA) and photoinitiators were infiltrated into pre-formed hBMSC-laden GH scaffolds and then photo-crosslinked. Results showed that infiltration of PEG at the beginning of chondrogenesis significantly increased the deposition of glycosaminoglycans (GAGs) in the central area of the scaffold. To explore the mechanism, we compared the cell migration and proliferation in the margin and central areas of GH and PEG-infiltrated GH scaffolds (GH+PEG). Limited cell migration was noticed in both groups, but more proliferating cells were observed in GH than in GH+PEG. Lastly, the in vitro repairing study with bovine cartilage explants showed that PEG- impregnated scaffolds integrated well with host tissues. These results indicate that PEG-GH hybrid scaffolds, created through infiltrating PEG into pre-formed GH scaffolds, display good integration capacity and represent a new tool for the repair of chondral injury.
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影响因子：
3.1
作者：
Farrell, Megan J.;Comeau, Eric S.;Mauck, Robert L.
通讯作者：
Mauck, Robert L.
影响因子：
10
作者：
Patel JM;Loebel C;Saleh KS;Wise BC;Bonnevie ED;Miller LM;Carey JL;Burdick JA;Mauck RL
通讯作者：
Mauck RL
影响因子：
3.7
作者：
Athens AA;Makris EA;Hu JC
通讯作者：
Hu JC
影响因子：
3.3
作者：
Kowalski, Michael A.;Fernandes, Lorenzo M.;Patel, Jay M.
通讯作者：
Patel, Jay M.
影响因子：
3.3
作者：
Lin, Hang;Beck, Angela M.;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.