Magnetic nanoparticles for biomedical NMR-based diagnostics.
Magnetic nanoparticles for biomedical NMR-based diagnostics.
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DOI：
10.3762/bjnano.1.17
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发表时间：
2010
影响因子：
3.1
通讯作者：
Lee H
中科院分区：
文献类型：
--
作者：
Shao H;Yoon TJ;Liong M;Weissleder R;Lee H
快速准确地测量生物样品中的蛋白质生物标志物、病原体和细胞可以为早期疾病诊断、治疗监测和个性化医疗设计提供有用的信息。一般来说，生物样品的磁化率可以忽略不计。因此，利用磁性纳米颗粒进行生物传感不仅提高了灵敏度，而且有效地减少了样品制备的需要。本文综述了基于磁共振效应的磁性纳米颗粒在体外生物分子和细胞检测中的应用。这种检测平台被称为诊断磁共振（DMR），利用磁性纳米颗粒作为接近传感器，调节分子靶向纳米颗粒周围水分子的自旋-自旋弛豫时间。通过开发更有效的磁性纳米粒子生物传感器，DMR对各种目标部分的检测限在过去几年中已经大大提高。目前，一个磁性纳米粒子文库已经开发出来，其中广泛的目标，包括DNA/mRNA，蛋白质，小分子/药物，细菌和肿瘤细胞，已经被量化。最近，随着小型化核磁共振探测器、更好的磁性纳米颗粒和新的共轭方法等新技术的发展，DMR技术的能力得到了进一步的发展。这些发展使得从小体积样品中进行平行和灵敏的测量成为可能。因此，DMR技术是一个非常有吸引力的便携、低成本和高效的生物医学环境中的生物分子检测平台。
Rapid and accurate measurements of protein biomarkers, pathogens and cells in biological samples could provide useful information for early disease diagnosis, treatment monitoring, and design of personalized medicine. In general, biological samples have only negligible magnetic susceptibility. Thus, using magnetic nanoparticles for biosensing not only enhances sensitivity but also effectively reduces sample preparation needs. This review focuses on the use of magnetic nanoparticles for in vitro detection of biomolecules and cells based on magnetic resonance effects. This detection platform, termed diagnostic magnetic resonance (DMR), exploits magnetic nanoparticles as proximity sensors, which modulate the spin–spin relaxation time of water molecules surrounding molecularly-targeted nanoparticles. By developing more effective magnetic nanoparticle biosensors, DMR detection limits for various target moieties have been considerably improved over the last few years. Already, a library of magnetic nanoparticles has been developed, in which a wide range of targets, including DNA/mRNA, proteins, small molecules/drugs, bacteria, and tumor cells, have been quantified. More recently, the capabilities of DMR technology have been further advanced with new developments such as miniaturized nuclear magnetic resonance detectors, better magnetic nanoparticles and novel conjugational methods. These developments have enabled parallel and sensitive measurements to be made from small volume samples. Thus, the DMR technology is a highly attractive platform for portable, low-cost, and efficient biomolecular detection within a biomedical setting.
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影响因子：
7.4
作者：
Koh, Isaac;Hong, Rui;Weissleder, Ralph;Josephson, Lee
通讯作者：
Josephson, Lee
影响因子：
16.6
作者：
Devaraj, Neal K.;Upadhyay, Rabi;Hatin, Jered B.;Hilderbrand, Scott A.;Weissleder, Ralph
通讯作者：
Weissleder, Ralph
影响因子：
16.6
作者：
Lee, Hakho;Yoon, Tae-Jong;Weissleder, Ralph
通讯作者：
Weissleder, Ralph
影响因子：
9.9
作者：
Fauconnier, N;Pons, JN;Bee, A
通讯作者：
Bee, A
影响因子：
3.3
作者：
GILLIS, P;KOENIG, SH
通讯作者：
KOENIG, SH