Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system.
Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system.
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DOI：
10.1186/1746-4811-9-39
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发表时间：
2013-10-11
期刊：
影响因子：
5.1
通讯作者：
Nekrasov V
中科院分区：
文献类型：
--
作者：
Belhaj K;Chaparro-Garcia A;Kamoun S;Nekrasov V
靶向基因组工程（也称为基因组编辑）已成为传统植物育种和转基因（GMO）方法的替代方法，以改善作物植物。直到最近，用于引入位点特异性双链DNA断裂的可用工具仅限于锌指核酸酶（ZFN）和TAL效应物核酸酶（TALEN）。然而，这些技术尚未被植物研究界广泛采用，这是由于每个靶基因的特异性DNA结合蛋白的复杂设计和费力组装。最近，基于细菌II型CRISPR（成簇的规则间隔短回文重复序列）/Cas（CRISPR相关）免疫系统出现了一种更简单的方法。CRISPR/Cas系统允许在可定制的小非编码RNA的引导下靶向切割基因组DNA，从而通过非同源末端连接（NHEJ）和同源定向修复（HDR）机制进行基因修饰。在本文中，我们总结并讨论了CRISPR/Cas技术在植物中的应用。
Targeted genome engineering (also known as genome editing) has emerged as an alternative to classical plant breeding and transgenic (GMO) methods to improve crop plants. Until recently, available tools for introducing site-specific double strand DNA breaks were restricted to zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs). However, these technologies have not been widely adopted by the plant research community due to complicated design and laborious assembly of specific DNA binding proteins for each target gene. Recently, an easier method has emerged based on the bacterial type II CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) immune system. The CRISPR/Cas system allows targeted cleavage of genomic DNA guided by a customizable small noncoding RNA, resulting in gene modifications by both non-homologous end joining (NHEJ) and homology-directed repair (HDR) mechanisms. In this review we summarize and discuss recent applications of the CRISPR/Cas technology in plants.
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影响因子：
13.8
作者：
D'Halluin K;Vanderstraeten C;Van Hulle J;Rosolowska J;Van Den Brande I;Pennewaert A;D'Hont K;Bossut M;Jantz D;Ruiter R;Broadhvest J
通讯作者：
Broadhvest J
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
46.9
作者：
Cho, Seung Woo;Kim, Sojung;Kim, Jin-Soo
通讯作者：
Kim, Jin-Soo
影响因子：
46.9
作者：
通讯作者：
--
通过反式编码的小 RNA 和宿主因子 RNase III 进行 CRISPR RNA 成熟。
影响因子：
64.8
作者：
Deltcheva, Elitza;Chylinski, Krzysztof;Sharma, Cynthia M.;Gonzales, Karine;Chao, Yanjie;Pirzada, Zaid A.;Eckert, Maria R.;Vogel, Joerg;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle