Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii.
Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii.
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从节节山羊草中克隆广泛有效的小麦叶锈病抗性基因 Lr42。
DOI：
10.1038/s41467-022-30784-9
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发表时间：
2022-06-01
影响因子：
16.6
通讯作者：
Liu, Sanzhen
中科院分区：
文献类型：
--
作者：
Lin, Guifang;Chen, Hui;Tian, Bin;Sehgal, Sunish K.;Singh, Lovepreet;Xie, Jingzhong;Rawat, Nidhi;Juliana, Philomin;Singh, Narinder;Shrestha, Sandesh;Wilson, Duane L.;Shult, Hannah;Lee, Hyeonju;Schoen, Adam William;Tiwari, Vijay K.;Singh, Ravi P.;Guttieri, Mary J.;Trick, Harold N.;Poland, Jesse;Bowden, Robert L.;Bai, Guihua;Gill, Bikram;Liu, Sanzhen
利用小麦野生近缘山羊草将Lr42抗叶锈病基因导入面包小麦。Lr42在苗期和成株期都具有抗性，对迄今测试的所有叶锈菌小种都广泛有效。Lr42在CIMMYT国际小麦育种计划中被广泛使用，所产生的品种在几个国家部署。在这里，使用批量分离RNA-Seq(BSR-Seq)定位策略，我们识别了Lr42的三个候选基因。富含亮氨酸重复序列(NLR)基因AET1Gv20040300的过表达诱导了小麦对叶锈病的强烈抗性，该基因的突变破坏了小麦对叶锈病的抗性。Lr42抗性等位基因在Ae中很少见。Tauschii，可能是异位重组所致。Lr42的克隆提供了诊断标记，已开发出1000多个携带Lr42的CIMMYT小麦品系，证明了Lr42在作物改良中的广泛应用和影响。节节麦抗叶锈病基因Lr42已被广泛应用于抗病小麦品种的选育，但其分子基础尚不清楚。在这里，作者通过在Ae中批量分离定位，证明了Lr42编码一个NLR型的抗病基因。并证实了其在普通小麦中的功能。
The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement. The Aegilops tauschii-derived leaf rust resistance gene Lr42 has been widely used for breeding resistance wheat cultivars, but the molecular basis is unknown. Here, the authors show that Lr42 encodes an NLR-type of disease resistance gene by bulked segregant mapping in Ae. tauschii and confirm its function in common wheat.
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影响因子：
46.9
作者：
Gaurav K;Arora S;Silva P;Sánchez-Martín J;Horsnell R;Gao L;Brar GS;Widrig V;John Raupp W;Singh N;Wu S;Kale SM;Chinoy C;Nicholson P;Quiroz-Chávez J;Simmonds J;Hayta S;Smedley MA;Harwood W;Pearce S;Gilbert D;Kangara N;Gardener C;Forner-Martínez M;Liu J;Yu G;Boden SA;Pascucci A;Ghosh S;Hafeez AN;O'Hara T;Waites J;Cheema J;Steuernagel B;Patpour M;Justesen AF;Liu S;Rudd JC;Avni R;Sharon A;Steiner B;Kirana RP;Buerstmayr H;Mehrabi AA;Nasyrova FY;Chayut N;Matny O;Steffenson BJ;Sandhu N;Chhuneja P;Lagudah E;Elkot AF;Tyrrell S;Bian X;Davey RP;Simonsen M;Schauser L;Tiwari VK;Randy Kutcher H;Hucl P;Li A;Liu DC;Mao L;Xu S;Brown-Guedira G;Faris J;Dvorak J;Luo MC;Krasileva K;Lux T;Artmeier S;Mayer KFX;Uauy C;Mascher M;Bentley AR;Keller B;Poland J;Wulff BBH
通讯作者：
Wulff BBH
影响因子：
5.1
作者：
Cloutier, Sylvie;McCallum, Brent D.;Jordan, Mark C.
通讯作者：
Jordan, Mark C.
影响因子：
2.3
作者：
COX, TS;RAUPP, WJ;GILL, BS
通讯作者：
GILL, BS
DOI：
10.3390/plants7020027
发表时间：
2018-03-28
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Alotaibi SS;Sparks CA;Parry MAJ;Simkin AJ;Raines CA
通讯作者：
Raines CA
影响因子：
56.9
作者：
Avni, Raz;Nave, Moran;Distelfeld, Assaf
通讯作者：
Distelfeld, Assaf