Atherosclerosis and Bone Loss in Humans-Results From Deceased Donors and From Patients Submitted to Carotid Endarterectomy.
Atherosclerosis and Bone Loss in Humans-Results From Deceased Donors and From Patients Submitted to Carotid Endarterectomy.
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死者捐助者和服从颈动脉内膜切除术的患者的人类造成的动脉粥样硬化和骨质流失。
DOI：
10.3389/fmed.2021.672496
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发表时间：
2021
影响因子：
3.9
通讯作者：
Santos MJ
中科院分区：
文献类型：
--
作者：
Carmona-Fernandes D;Barreira SC;Leonardo N;Casimiro RI;Castro AM;Santos PO;Fernandes AN;Cortes-Figueiredo F;Gonçalves CA;Cruz R;Fernandes ML;Ivo M;Pedro LM;Canhão H;Fonseca JE;Santos MJ
背景和目的：动脉粥样硬化和骨质疏松症有共同的危险因素，以及炎症机制。我们的目的是了解动脉粥样硬化病变是如何与骨骼紊乱相关的。研究方法：分析了45例死亡供体动脉和骨中促炎和骨代谢相关蛋白（IL-1β、IL-6、IL-17 A、TNF、RANKL、OPG、COL 1、CTSK、OCL、TRAP、CBFA 1、DKK 1、SOST、ADIPOQ和ADIPOR 1）的基因表达，并将脂肪组织用作对照。此外，在139例接受颈动脉内膜切除术的晚期动脉粥样硬化患者中，我们比较了骨密度正常和低骨密度患者的钙含量（茜素红）和斑块炎症评分（CD 3+，CD 68+和脂联素），并探讨了动脉粥样硬化斑块中基因表达与骨密度之间的关系。在供体和患者中测量促炎和骨相关蛋白的血清水平。相关性采用Pearson或斯皮尔曼相关性检验进行研究，合理时进行多变量回归分析。结果如下：骨重建和促炎蛋白的基因表达在骨和主动脉中呈正相关，与供体的年龄和性别无关，但在脂肪组织中不相关。骨形成基因的表达在动脉内膜切除患者的动脉粥样硬化斑块中显著更高，这些患者具有正常与低BMD以及炎性CD 68+评分，与患者的年龄和性别无关，但与体重指数无关。未观察到血清水平与促炎蛋白或骨重塑蛋白的基因表达水平之间的关系。结论：我们的研究结果表明，在动脉粥样硬化性疾病和骨质疏松症的背景下，骨和血管之间的关系可能依赖于所涉及的组织之间的内在联系，独立于疾病阶段。促炎蛋白和骨重塑蛋白的血清测量不能准确地解释组织病理过程。
Background and Aims: Atherosclerosis and osteoporosis share common risk factors, as well as inflammatory mechanisms. Our aim was to understand how atherosclerotic lesions are related with disturbances in bone. Methods: Gene expression of pro-inflammatory and bone metabolism related proteins (IL-1β, IL-6, IL-17A, TNF, RANKL, OPG, COL1, CTSK, OCL, TRAP, CBFA1, DKK1, SOST, ADIPOQ, and ADIPOR1) were analyzed in arteries and bones from 45 deceased donors and adipose tissue was used as control. Additionally, in 139 patients with advanced atherosclerosis submitted to carotid endarterectomy we compared calcium content (Alizarin red) and plaque inflammatory scores (CD3+, CD68+, and adiponectin) of patients with normal bone mineral density (BMD) with those with low BMD and explored the associations between gene expression in atherosclerotic plaques and BMD. Serum levels of pro-inflammatory and bone related proteins were measured both in donors and patients. Associations were investigated by the Pearson or Spearman correlation tests, and multivariate regression analyzes were performed when justified. Results: Gene expression of bone remodeling and pro-inflammatory proteins correlated positively in bone and aorta, independently of age and sex of donors, but not in adipose tissue. The expression of bone formation genes was significantly higher in atheroma plaques from endarterectomized patients with normal vs. low BMD as well as inflammatory CD68+ scores, regardless of patients' age and sex, but not of body mass index. No relationship was observed between serum levels and gene expression levels of pro-inflammatory or bone remodeling proteins. Conclusions: Our results suggest that the relationship between bones and vessels in the context of atherosclerotic disease and osteoporosis may rely on the intrinsic connection between the tissues involved, independently of disease stage. Serum measurements of pro-inflammatory and bone-remodeling proteins do not accurately translate tissue pathologic processes.
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影响因子：
2.2
作者：
Miyazaki T;Tokimura F;Tanaka S
通讯作者：
Tanaka S
影响因子：
3.5
作者：
Pietschmann, Peter;Rauner, Martina;Kerschan-Schindl, Katharina
通讯作者：
Kerschan-Schindl, Katharina
DOI：
10.1007/978-1-61779-415-5_17
发表时间：
2012-01-01
期刊：
BONE RESEARCH PROTOCOLS, SECOND EDITION
影响因子：
--
作者：
Hughes, Alun;Stewart, Tracy L.;Mann, Val
通讯作者：
Mann, Val
影响因子：
3.7
作者：
Cascão R;Vidal B;Lopes IP;Paisana E;Rino J;Moita LF;Fonseca JE
通讯作者：
Fonseca JE
DOI：
10.1097/01.asn.0000129337.50739.48
发表时间：
2004-07-01
影响因子：
13.6
作者：
London, GM;Marty, C;De Vernejoul, MC
通讯作者：
De Vernejoul, MC