Glycosaminoglycan concentration in synovium and other tissues of rabbit knee in relation to synovial hydraulic resistance.
Glycosaminoglycan concentration in synovium and other tissues of rabbit knee in relation to synovial hydraulic resistance.
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兔膝滑膜和其他组织中的糖胺聚糖浓度与滑膜水阻力的关系。
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
R. Mason
中科院分区：
文献类型：
--
作者：
F. Price;J. Levick;R. Mason
1.关节滑膜衬里的液压阻力是滑液周转中的关键耦合系数，被认为取决于滑膜细胞间隙中的生物聚合物（糖胺聚糖（GAG）和胶原）的浓度，因为这些聚合物产生液压阻力。本研究的主要目的是从8只家兔膝关节中获得显微镜下分离的极薄滑膜毫克样本，并定量分析这些样本中的GAG（硫酸软骨素、硫酸乙酰肝素和透明质酸）和胶原蛋白，以便与已发表的液压阻力数据进行比较。还研究了滑液和股骨软骨。2.滑膜包含73 +/-3%重量的水（平均值+/-S.E.M.）。在每克湿组织的270毫克固体中，形成136毫克蛋白质（通过自动氨基酸分析），其中94毫克是通过羟脯氨酸分析的胶原蛋白。由胶原质量和原纤维体积分数（0.153的组织，通过形态测定）计算出原纤维比容为1.43ml/g分子胶原，并且原纤维水含量为47体积%。3.通过毛细管区带电泳测量的4-硫酸软骨素（C4S）和6-硫酸软骨素（C6S）的浓度为0.55 mg/g滑膜-远高于滑液（0.04 mg g-1），远低于软骨（27.8 mg g-1）。滑膜中的C4S/C6S比值（7.3）与软骨中的C4S/C6S比值（0.7）不同，表明滑膜中不同的蛋白多糖占主导地位。通过放射性钌红结合测定的硫酸乙酰肝素浓度为0.92 mg/g滑膜（滑液，0.08 mg g-1;软骨，0.72 mg g-1）。4.与硫酸化GAG相比，滑液中透明质酸浓度最高（3.53 mg g-1;生物素化G1结构域结合试验）。尽管滑膜和滑液之间存在开放接触，但滑膜中的浓度仅为0.56 mg g-1（经间质体积分数校正，0.66）。这可能是由于排除或洗脱。5.总GAG质量约为4 mg/g滑液囊。经滑膜流动模型表明，4 mg g-1的均匀GAG浓度小于解释滑膜液压阻力实验估计值所需浓度的1/3。阻力估计的误差似乎不足以解决这个问题。因此，有可能是，额外的聚合物材料，如糖蛋白和蛋白聚糖核心蛋白，可能有助于液压阻力。
1. The hydraulic resistance of the synovial lining of a joint, a key coupling coefficient in synovial fluid turnover, is thought to depend on the concentration of biopolymers (glycosaminoglycans (GAGs) and collagen) in the synovial intercellular spaces, because these polymers create hydraulic drag. The primary aim of this study was to obtain microscopically separated, milligram samples of the very thin synovium from eight rabbit knees, and to analyse these quantitatively for GAGs (chondroitin sulphate, heparan sulphate and hyaluronan) and collagen to allow comparison with published hydraulic resistance data. Synovial fluid and femoral cartilage were also studied. 2. Synovium comprised 73 +/‐ 3% water by weight (mean +/‐ S.E.M.). Of the 270 mg solid per gram of wet tissue, protein formed 136 mg (by automated amino acid analysis), and of this 94 mg was collagen by hydroxyproline analysis. From the collagen mass and fibril volume fraction (0.153 of tissue by morphometry), fibrillar specific volume was calculated to be 1.43 ml per gram of molecular collagen, and fibril water content 47% by volume. 3. The concentration of chondroitin 4‐sulphate (C4S) plus chondroitin 6‐sulphate (C6S), measured by capillary zone electrophoresis was 0.55 mg per gram of synovium‐‐much greater than in synovial fluid (0.04 mg g‐1) and much less than in cartilage (27.8 mg g‐1). The C4S/C6S ratio in synovium (7.3) differed from that in cartilage (0.7), indicating that different proteoglycans predominated in synovium. The heparan sulphate concentration, assayed by radioactive Ruthenium Red binding, was 0.92 mg per gram of synovium (synovial fluid, 0.08 mg g‐1; cartilage, 0.72 mg g‐1). 4. In contrast to sulphated GAGs, the hyaluronan concentration was highest in synovial fluid (3.53 mg g‐1; biotinylated G1 domain binding assay). The concentration in synovial interstitium was only 0.56 mg g‐1 (corrected for interstitial volume fraction, 0.66), even though there is open contact between synovial interstitium and synovial fluid. This may be due to exclusion or washout. 5. Total GAG mass was approximately 4 mg per gram of synovial interstitium. A model of trans‐synovial flow indicated that a uniform GAG concentration of 4 mg g‐1 is less than 1/3rd of that required to explain the experimental estimate of synovial hydraulic resistance. Errors in the resistance estimate do not appear to be large enough to resolve the problem. It is possible, therefore, that additional polymeric material in the interstitium, such as glycoproteins and proteoglycan core protein, may contribute to the hydraulic resistance.
103钌红，一种用于检测纳克级糖胺聚糖的试剂。
影响因子：
2.9
作者：
Carlson,SS
通讯作者：
Carlson,SS
犬滑膜细胞蛋白聚糖和连接蛋白之间相互作用的证据。
DOI：
10.1016/0304-4165(85)90144-8
发表时间：
1985
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Fife,RS;Myers,SL
通讯作者：
Myers,SL