Thauera sinica sp. nov., a phenol derivative-degrading bacterium isolated from activated sludge
Thauera sinica sp. nov., a phenol derivative-degrading bacterium isolated from activated sludge
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中华托乌拉
DOI：
10.1007/s10482-017-0993-5
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发表时间：
2017-12
影响因子：
2.6
通讯作者：
Liu Jianguo
中科院分区：
文献类型：
--
作者：
Qiao Nenghu;Xi Lijun;Zhang Jingjing;Liu Dejian;Ge Baosheng;Liu Jianguo
从中国某污水处理厂的活性污泥中分离到一株能降解苯酚衍生物的菌株K11T。该菌株能降解十余种苯酚衍生物，经鉴定为革兰氏染色阴性、杆状、无孢子型兼性厌氧细菌，鞭毛极强。该菌株在0-2.5%(w/v)的NaC l(最适0-1%)、4-43℃(最适30-35℃)、pH 4.5-10.5(最适7.5-8)的大豆胰酶发酵液中生长。对几乎全长的16S rRNA基因序列的比较分析表明，该菌株属于多伊拉属。16S rRNA基因序列与氯苯沙门氏菌3CB-1T有很高的相似性(97.5%)，与其他公认的沙伊拉菌株的相似性较低。该菌株DNA的G+C含量为67.8mol%。K11T与芳香斑潜蝇DSM6984T的DNA-DNA杂交值为10.4±4.5%。K11T与其他9个模式菌株的基因组同源性均小于81.1%。对菌株K11的化学分类分析表明，Q-8是主要的苯二酚；极性脂含有磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺、2种未知磷脂和5种未知脂类；主要细胞脂肪酸鉴定为总和特征3(C16：1ω7c和/或iso-C15：02-OH；45.9%)，其次是C16：0(20.5%)和C18：1ω7c(15.8%)。根据表型和系统发育、DNA-DNA杂交、OthoANI、化学分类和生理生化试验结果，将其命名为一新种，命名为Thauera Sinica sp.11月菌株K11T(=CGMCC 1.15731T=KACC 19216T)为模式菌株。
A bacterial strain, K11T, capable of degrading phenol derivatives was isolated from activated sludge of a sewage treatment plant in China. This strain, which can degrade more than ten phenol derivatives, was identified as a Gram-stain negative, rod-shaped, asporogenous, facultative anaerobic bacterium with a polar flagellum. The strain was found to grow in tryptic soy broth in the presence of 0-2.5% (w/v) NaCl (optimum 0-1%), at 4-43 °C (optimum 30-35 °C) and pH 4.5-10.5 (optimum 7.5-8). Comparative analysis of nearly full-length 16S rRNA gene sequences showed that this strain belongs to the genus Thauera. The 16S rRNA gene sequence was found to show high similarity (97.5%) to that of Thauera chlorobenzoica 3CB-1T, with lesser similarity to other recognised Thauera strains. The G+C content of the DNA of the strain was determined to be 67.8 mol%. The DNA-DNA hybridization value between K11Tand Thauera aromatica DSM6984Twas 10.4 ± 4.5%. The genomic OrthoANI values of K11Twith the other nine type strains of genus Thauera were less than 81.1%. Chemotaxonomic analysis of strain K11Trevealed that Q-8 is the predominant quinone; the polar lipids contain phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and five uncharacterised lipids; the major cellular fatty acid was identified as summed feature 3 (C16:1ω7c and/or iso-C15:02-OH; 45.9%), followed by C16:0(20.5%) and C18:1ω7c (15.8%). Based on the phenotypic and phylogenetic evidence, DNA-DNA hybridisation, OrthoANI, chemotaxonomic analysis and results of the physiological and biochemical tests, a new species named Thauera sinica sp. nov. is proposed with strain K11T(= CGMCC 1.15731T= KACC 19216T) designated as the type strain.
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DOI：
10.1007/s10482-016-0770-x
发表时间：
2017
影响因子：
2.6
作者：
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影响因子：
2.1
作者：
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通讯作者：
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DOI：
10.1007/s10482-017-0841-7
发表时间：
2017-02
期刊：
Antonie van Leeuwenhoek
影响因子：
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作者：
R. Rosselló-Móra;M. Trujillo;I. Sutcliffe
通讯作者：
R. Rosselló-Móra;M. Trujillo;I. Sutcliffe
影响因子：
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作者：
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通讯作者：
Sayler, GS
DOI：
10.4028/www.scientific.net/amr.356-360.44
发表时间：
2011-10
期刊：
Advanced Materials Research
影响因子：
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作者：
Zi-jun Xiao;Fang-Fang Huo-Fang;R. Qi;Yunling Huang
通讯作者：
Zi-jun Xiao;Fang-Fang Huo-Fang;R. Qi;Yunling Huang