Clustering and conservation patterns of human microRNAs.
Clustering and conservation patterns of human microRNAs.
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DOI：
10.1093/nar/gki567
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发表时间：
2005
影响因子：
14.9
通讯作者：
Margalit H
中科院分区：
文献类型：
--
作者：
Altuvia Y;Landgraf P;Lithwick G;Elefant N;Pfeffer S;Aravin A;Brownstein MJ;Tuschl T;Margalit H
MicroRNAs （miRNAs）是一种长约22个nt-long的非编码RNA分子，被认为在基因调控中发挥重要作用。我们提出了一个全面的分析，在人类已知的mirna的保护和聚类模式。我们发现人类miRNA基因聚类显著高于随机预期。在本研究中分析的已知人类miRNA基因中，总共有37%出现在两个或更多的染色体集群中，染色体对对距离最多为3000 nt。将其他四种生物的miRNA序列与其同源物进行比较，揭示了典型的保守模式，持续存在于整个集群中。此外，与随机基因组区域相比，我们在已知miRNA基因附近发现了典型的保守模式和其他miRNA样特性的富集。这可能意味着在这些区域存在其他未知的mirna，这与目前的认识一致，即存在被忽视的mirna。事实上，通过将我们的预测与克隆结果和其他哺乳动物中已鉴定的miRNA基因进行比较，我们证实了在先前已知的miRNA基因附近另外18个人类miRNA基因的预测。我们的研究将小于3000 nt的人类mirna聚集的比例提高到42%。这表明miRNA基因的聚类比目前所认为的要高，暗示其进化和功能意义。
MicroRNAs (miRNAs) are ∼22 nt-long non-coding RNA molecules, believed to play important roles in gene regulation. We present a comprehensive analysis of the conservation and clustering patterns of known miRNAs in human. We show that human miRNA gene clustering is significantly higher than expected at random. A total of 37% of the known human miRNA genes analyzed in this study appear in clusters of two or more with pairwise chromosomal distances of at most 3000 nt. Comparison of the miRNA sequences with their homologs in four other organisms reveals a typical conservation pattern, persistent throughout the clusters. Furthermore, we show enrichment in the typical conservation patterns and other miRNA-like properties in the vicinity of known miRNA genes, compared with random genomic regions. This may imply that additional, yet unknown, miRNAs reside in these regions, consistent with the current recognition that there are overlooked miRNAs. Indeed, by comparing our predictions with cloning results and with identified miRNA genes in other mammals, we corroborate the predictions of 18 additional human miRNA genes in the vicinity of the previously known ones. Our study raises the proportion of clustered human miRNAs that are <3000 nt apart to 42%. This suggests that the clustering of miRNA genes is higher than currently acknowledged, alluding to its evolutionary and functional implications.
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影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
Tuschl, T
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S
影响因子：
10.5
作者：
Mourelatos, Z;Dostie, J;Dreyfuss, G
通讯作者：
Dreyfuss, G
影响因子：
56.9
作者：
Lim, LP;Glasner, ME;Bartel, DP
通讯作者：
Bartel, DP