Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals.
Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals.
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DOI：
10.1016/j.ymeth.2007.01.006
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发表时间：
2007-08
期刊：
影响因子：
4.8
通讯作者：
Kumarasamy, Yashodharan
中科院分区：
文献类型：
--
作者：
Sarker, Satyajit D.;Nahar, Lutfun;Kumarasamy, Yashodharan
Drummond 和 Waigh 在 2000 年描述的利用微量滴定板的刃天青测定法经过修改，可以更准确地测定天然产物的最低抑菌浓度 (MIC) 值，包括针对各种细菌菌株的粗提取物、色谱级分或纯化化合物。这种改良的刃天青方法简单、灵敏、快速、稳健、可靠，可成功用于评估天然产物的抗菌特性。
The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, robust and reliable, and could be used successfully to assess antibacterial properties of natural products.
影响因子：
5.2
作者：
Koeth, LM;King, A;Poupard, JA
通讯作者：
Poupard, JA
影响因子：
5.4
作者：
Kumarasamy, Y;Cox, PJ;Sarker, SD
通讯作者：
Sarker, SD
影响因子：
12.8
作者：
McNicholl, Bridget P.;McGrath, John W.;Quinn, John P.
通讯作者：
Quinn, John P.