Helicobacter pylori cagA 12-bp insertion can be a marker for duodenal ulcer in Okinawa, Japan.
Helicobacter pylori cagA 12-bp insertion can be a marker for duodenal ulcer in Okinawa, Japan.
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DOI：
10.1111/jgh.12067
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发表时间：
2013-02
影响因子：
4.1
通讯作者：
Yamaoka Y
中科院分区：
文献类型：
--
作者：
Matsuo Y;Shiota S;Matsunari O;Suzuki R;Watada M;Binh TT;Kinjo N;Kinjo F;Yamaoka Y
幽门螺杆菌（Helicobacter pylori，Hp）cagA基因3′端的重复序列主要分为东亚型和西方型两种。最近的研究表明，来自日本冲绳的菌株中的Western型cagA形成了一个不同的簇（J-Western-type cagA亚型）。我们还报道了J-Western型cagA在cagA序列的5′端有一个12 bp的插入片段。冲绳和美国cagA中12-bp插入的流行率通过DNA测序进行检测。然后，我们设计了一对引物，可以检测12-bp的插入，只有通过聚合酶链反应（PCR）。用PCR方法检测了336株分离自冲绳的12-bp插入菌株的流行情况。在西方型cagA/vacA s1 m2菌株的情况下，12-bp插入的流行率在分离自冲绳的菌株中显著高于来自美国的菌株（P = 0.002）。系统进化树分析表明，插入12 bp片段的菌株在J-Western-type cagA亚型中形成两个独立的簇，一个来自冲绳，另一个来自美国。我们设计的引物对冲绳的敏感性（100%）和特异性（90.8%）都很高。在冲绳的十二指肠溃疡（DU）、胃炎、胃癌（GC）和胃溃疡（GU）菌株中分别发现23.7%、14.3%、4.2%和4.0%的12-bp插入（DU vs. GU P < 0.001）。虽然机制尚不清楚，但12-bp插入的存在与DU的存在有关，并可能对GU和GC具有抑制作用。
Helicobacter pylori cagA can be classified into mainly two types (East-Asian-type and Western-type cagA) according to the repeat regions located in the 3′ region. Recent studies showed that the Western-type cagA in strains from Okinawa, Japan formed a different cluster (J-Western-type cagA subtype). We also reported that J-Western-type cagA possess a 12-bp insertion located in the 5′ region of cagA sequence. The prevalence of 12-bp insertion in cagA in Okinawa and the United States (U.S.) was examined by DNA sequencing. We then designed the primer pair which can detect the 12-bp insertion only by polymerase chain reaction (PCR). The prevalence of strains with 12-bp insertion was examined in 336 strains isolated from Okinawa by PCR. In case of Western-type cagA/vacA s1m2 strains, the prevalence of 12-bp insertion was significantly higher in strains isolated from Okinawa than that from the U.S. (P = 0.002). Phylogenetic tree showed that strains with 12-bp insertion formed two individual clusters within J-Western-type cagA subtype; one is from Okinawa and another is from the U.S. Our designed primer set showed high sensitivity (100%) and specificity (90.8%) in Okinawa. The 12-bp insertion was found in 23.7%, 14.3%, 4.2%, and 4.0% of strains with duodenal ulcer (DU), gastritis, gastric cancer (GC), and gastric ulcer (GU), respectively (P < 0.001 for DU vs. GU) in Okinawa. Although the mechanisms are unknown, the presence of 12-bp insertion was associated with the presence of DU and might have a suppressive action on GU and GC.
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影响因子：
6.3
作者：
Satomi, Satoko;Yamakawa, Akiyo;Azuma, Takeshi
通讯作者：
Azuma, Takeshi
DOI：
10.1073/pnas.90.12.5791
发表时间：
1993-06-15
影响因子：
11.1
作者：
COVACCI, A;CENSINI, S;RAPPUOLI, R
通讯作者：
RAPPUOLI, R
影响因子：
3.2
作者：
Duncan, Stacy S.;Valk, Pieter L.;Cover, Timothy L.
通讯作者：
Cover, Timothy L.
影响因子：
9.4
作者：
Bui Xuan Truong;Vo Thi Chi Mai;Azuma, Takeshi
通讯作者：
Azuma, Takeshi
影响因子：
7.6
作者：
Nobuta, A;Asaka, M;Shinomura, J
通讯作者：
Shinomura, J