Topical antibiotics reduce CD11c+ cell numbers in the healthy murine cornea and modulate their response to contact lens wear.
Topical antibiotics reduce CD11c+ cell numbers in the healthy murine cornea and modulate their response to contact lens wear.
复制标题
DOI：
10.1038/s41598-022-14847-x
复制
发表时间：
2022-06-23
影响因子：
4.6
通讯作者：
Fleiszig SMJ
中科院分区：
文献类型：
--
作者：
Datta A;Lee J;Truong T;Evans DJ;Fleiszig SMJ
之前我们报道过隐形眼镜在健康小鼠角膜中诱导的CD11c+细胞反应，这一现象也发生在人类中。为了测试眼部相关细菌的受累情况，研究了在晶状体佩戴24小时内，局部抗生素对角膜CD11c+细胞群的影响。采用庆大霉素联合氧氟沙星（0.3%）或单独使用庆大霉素治疗，部分患者在配戴晶状体前（24 h）进行治疗。对侧pbs治疗眼作为对照。CD11c- yfp（黄色荧光蛋白）小鼠允许CD11c+细胞可视化。使用FISH （16S rrna靶向探针）或click-chemistry （alkDala）标记眼表或隐形眼镜上的活菌。抗生素治疗降低了无晶状体磨损的基线CD11c+细胞数量，如果角膜在磨损期间进行预处理和治疗，则抑制了CD11c+细胞对晶状体磨损的反应。在任何情况下，很少有细菌在角膜或晶状体上定植。无论是否佩戴晶状体，抗生素都能显著降低结膜共生菌，但仅佩戴晶状体对结膜共生菌的影响最小。故意接种结膜共生菌可触发CD11c+细胞反应，而不考虑抗生素预处理。这些结果表明，虽然晶状体佩戴并不一定会增加结膜共生体的可量化数量，但抗生素中和的共生体在晶状体相关的CD11c+细胞反应和维持基线CD11c+细胞群中发挥作用。
Previously we reported contact lens-induced CD11c+ cell responses in healthy mouse corneas, a phenomenon that also occurs in humans. To test involvement of ocular-associated bacteria, the impact of topical antibiotics on corneal CD11c+ cell populations during 24 h of lens wear was examined. Corneas were treated with gentamicin and ofloxacin (0.3%) or gentamicin alone, some also treated prior to lens wear (24 h). Contralateral PBS-treated eyes served as controls. CD11c-YFP (Yellow Fluorescent Protein) mice allowed CD11c+ cell visualization. Viable bacteria, on the ocular surface or contact lens, were labeled using FISH (16S rRNA-targeted probe) or click-chemistry (alkDala). Antibiotic treatment reduced baseline CD11c+ cell numbers without lens wear and suppressed CD11c+ cell responses to lens wear if corneas were both pretreated and treated during wear. Few bacteria colonized corneas or lenses under any circumstances. Conjunctival commensals were significantly reduced by antibiotics with or without lens wear, but minimally impacted by lens wear alone. Deliberate inoculation with conjunctival commensals triggered CD11c+ cell responses irrespective of antibiotic pretreatment. These results suggest that while lens wear does not necessarily increase quantifiable numbers of conjunctival commensals, those neutralized by antibiotics play a role in lens-associated CD11c+ cell responses and maintaining baseline CD11c+ cell populations.
登录
查看更多内容
微生物群对铜绿假单胞菌诱导的角膜炎的抗性。
影响因子：
6.7
作者：
Kugadas A;Christiansen SH;Sankaranarayanan S;Surana NK;Gauguet S;Kunz R;Fichorova R;Vorup-Jensen T;Gadjeva M
通讯作者：
Gadjeva M
影响因子：
4.6
作者：
Metruccio MME;Tam C;Evans DJ;Xie AL;Stern ME;Fleiszig SMJ
通讯作者：
Fleiszig SMJ
影响因子：
6.4
作者：
Collin M;McGovern N;Haniffa M
通讯作者：
Haniffa M
影响因子：
3.1
作者：
Gauguet, Stefanie;D'Ortona, Samantha;Pier, Gerald B.
通讯作者：
Pier, Gerald B.
影响因子：
9.4
作者：
FLEISZIG, SMJ;EFRON, N
通讯作者：
EFRON, N