Geometric control of human stem cell morphology and differentiation.
Geometric control of human stem cell morphology and differentiation.
复制标题
人类干细胞形态和分化的几何控制。
DOI：
10.1039/c0ib00016g
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发表时间：
2010-08
期刊：
影响因子：
--
通讯作者：
Vunjak-Novakovic G
中科院分区：
文献类型：
--
作者：
Wan LQ;Kang SM;Eng G;Grayson WL;Lu XL;Huo B;Gimble J;Guo XE;Mow VC;Vunjak-Novakovic G
在组织形态发生过程中，干细胞和祖细胞迁移、增殖和分化，细胞形状、大小和机械应力发生显著变化。局部细胞功能取决于细胞因子的空间分布以及细胞所在的局部机械微环境。在这项研究中，我们利用微模式技术控制人脂肪干细胞的组织，研究多细胞形态对细胞分化早期细胞功能空间分布的影响。通过药物治疗探讨了细胞骨架张力的潜在作用。我们的研究结果表明，干细胞在几何图案上的培养导致了图案和位置特异性的细胞形态、增殖和分化。细胞增殖最高的区域出现在大的、扩散的细胞区域（如环的外缘和矩形的短边）。相反，干细胞分化与含有小而细长细胞的区域（如环的内缘和矩形短边附近的区域）共定位。抑制肌动球蛋白形成的药物的应用导致缺乏几何特异性分化模式。本研究通过相关的物理力证实了底物几何对干细胞分化的作用，为研究细胞功能的生物物理调控提供了一个简单可控的系统。
During tissue morphogenesis, stem cells and progenitor cells migrate, proliferate, and differentiate, with striking changes in cell shape, size, and acting mechanical stresses. The local cellular function depends on the spatial distribution of cytokines as well as local mechanical microenvironments in which the cells reside. In this study, we controlled the organization of human adipose derived stem cells using micro-patterning technologies, to investigate the influence of multi-cellular form on spatial distribution of cellular function at an early stage of cell differentiation. The underlying role of cytoskeletal tension was probed through drug treatment. Our results show that the cultivation of stem cells on geometric patterns resulted in pattern- and position-specific cell morphology, proliferation and differentiation. The highest cell proliferation occurred in the regions with large, spreading cells (such as the outer edge of a ring and the short edges of rectangles). In contrast, stem cell differentiation co-localized with the regions containing small, elongated cells (such as the inner edge of a ring and the regions next to the short edges of rectangles). The application of drugs that inhibit the formation of actomyosin resulted in the lack of geometrically specific differentiation patterns. This study confirms the role of substrate geometry on stem cell differentiation, through associated physical forces, and provides a simple and controllable system for studying biophysical regulation of cell function.
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影响因子：
4
作者：
Freytes, Donald O.;Wan, Leo Q.;Vunjak-Novakovic, Gordana
通讯作者：
Vunjak-Novakovic, Gordana
DOI：
10.1038/jid.2008.265
发表时间：
2009-03
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1016/0309-1651(91)90146-a
发表时间：
1991-10-01
期刊：
CELL BIOLOGY INTERNATIONAL REPORTS
影响因子：
--
作者：
ISEMURA, M;MITA, T;MOTOMIYA, M
通讯作者：
MOTOMIYA, M
影响因子：
4.8
作者：
Kovács, M;Tóth, J;Sellers, JR
通讯作者：
Sellers, JR
影响因子：
2
作者：
HARRIS, AK
通讯作者：
HARRIS, AK