Inactivation of pathogenic viruses by plant-derived tannins: strong effects of extracts from persimmon (Diospyros kaki) on a broad range of viruses.
Inactivation of pathogenic viruses by plant-derived tannins: strong effects of extracts from persimmon (Diospyros kaki) on a broad range of viruses.
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DOI：
10.1371/journal.pone.0055343
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sakaguchi T
中科院分区：
文献类型：
--
作者：
Ueda K;Kawabata R;Irie T;Nakai Y;Tohya Y;Sakaguchi T
单宁，植物来源的多酚和其他相关化合物，已经在许多领域，如食品工业和制造业中使用了很长一段时间。在这项研究中，我们研究了单宁对12种不同病毒的抗病毒作用，包括包膜病毒（流感病毒H3 N2，H5 N3，单纯疱疹病毒-1，水泡性口炎病毒，仙台病毒和纽卡斯尔病病毒）和无包膜病毒（脊髓灰质炎病毒，柯萨奇病毒，腺病毒，轮状病毒，猫杯状病毒和小鼠诺如病毒）。我们发现，柿（柿），其中含有约。22%的柿子单宁，对所有测试病毒的病毒感染性降低超过4个对数，对广泛的病毒显示出强大的抗病毒作用。其他来自绿色茶、金合欢和五倍子的单宁对某些病毒有效，而咖啡提取物对任何病毒都无效。然后，我们主要通过使用流感病毒来研究柿子提取物的抗病毒作用的机制。Perceptide提取物在0.25%的浓度下在30秒内有效，并抑制病毒与细胞的附着。在病毒感染前用柿子提取物预处理细胞或在病毒感染后用柿子提取物后处理细胞不抑制病毒复制。蛋白质聚集似乎是柿子单宁的抗病毒作用的基本机制，因为当纯化的病毒体用柿子提取物处理时，病毒蛋白形成聚集体，并且因为抗病毒作用被非特异性蛋白质牛血清白蛋白竞争性抑制。考虑到柿子单宁是一种食品补充剂，它有可能被用作一种安全和高效的抗病原病毒的抗病毒试剂。
Tannins, plant-derived polyphenols and other related compounds, have been utilized for a long time in many fields such as the food industry and manufacturing. In this study, we investigated the anti-viral effects of tannins on 12 different viruses including both enveloped viruses (influenza virus H3N2, H5N3, herpes simplex virus-1, vesicular stomatitis virus, Sendai virus and Newcastle disease virus) and non-enveloped viruses (poliovirus, coxsachievirus, adenovirus, rotavirus, feline calicivirus and mouse norovirus). We found that extracts from persimmon (Diospyros kaki), which contains ca. 22% of persimmon tannin, reduced viral infectivity in more than 4-log scale against all of the viruses tested, showing strong anti-viral effects against a broad range of viruses. Other tannins derived from green tea, acacia and gallnuts were effective for some of the viruses, while the coffee extracts were not effective for any of the virus. We then investigated the mechanism of the anti-viral effects of persimmon extracts by using mainly influenza virus. Persimmon extracts were effective within 30 seconds at a concentration of 0.25% and inhibited attachment of the virus to cells. Pretreatment of cells with the persimmon extracts before virus infection or post-treatment after virus infection did not inhibit virus replication. Protein aggregation seems to be a fundamental mechanism underlying the anti-viral effect of persimmon tannin, since viral proteins formed aggregates when purified virions were treated with the persimmon extracts and since the anti-viral effect was competitively inhibited by a non-specific protein, bovine serum albumin. Considering that persimmon tannin is a food supplement, it has a potential to be utilized as a safe and highly effective anti-viral reagent against pathogenic viruses.
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