The history, state of the art and future prospects for oleaginous yeast research.
The history, state of the art and future prospects for oleaginous yeast research.
复制标题
DOI：
10.1186/s12934-021-01712-1
复制
发表时间：
2021-12-07
影响因子：
6.4
通讯作者：
Chuck CJ
中科院分区：
文献类型：
--
作者：
Abeln F;Chuck CJ
生物柴油和加氢处理酯等脂类生物燃料是减少运输部门对环境影响的全球倡议的核心部分。目前，绝大多数产品来自第一代原料，如菜籽油和废食用油。然而，大豆油和棕榈油开采量的增加导致了大规模的森林砍伐，烟雾排放，并严重影响了热带地区的生物多样性。一个有希望的替代品，可能能够满足未来的需求可持续地，是产油酵母。尽管已经有143年的历史，但在过去的十年中，人们越来越努力地开发一个可行的工业系统，目前每年发表约100篇研究论文。在学术文献中，据报道约有160种天然酵母在富含甘油酯的油中产生超过其干重的20%。研究最深入的产油酵母是产油皮丝孢酵母（20%的出版物）、圆红酵母（19%）和解脂耶氏酵母（19%）。产油酵母主要生长在单一底物（60%）、水解产物（26%）或甘油（19%）上，并且主要以mL规模（66%）生长。工艺开发和基因改造（7%）已被应用于改变酵母的性能和脂质，用于生产生物燃料（77%），食品/补充剂（24%），油脂化学品（19%）或动物饲料（3%）。尽管经过了世纪的研究和最近先进的基因工程技术的应用，但经济上可行的商品油替代品的工业生产仍然是难以捉摸的。这主要是由于估计的高生产成本，然而，在21世纪，气候变化将彻底改变全球粮食供应网络，政府可能会对更具破坏性的作物采取直接行动，酵母脂质为本地化，可持续的脂质生产提供了一个灵活的平台。基于大多数产油酵母学术出版物的数据，这篇综述是对产油酵母研究历史的指导，对迄今为止实现的最佳生长和脂质生产的评估，对工业生产采用的各种策略，以及重要的是，关于需要建立在这个庞大的工作基础上的关键讨论，以使生产酵母衍生的，更可持续的，甘油酯油的商业现实。在线版本包含补充材料，可通过10.1186/s12934-021-01712-1获得。
Lipid-based biofuels, such as biodiesel and hydroprocessed esters, are a central part of the global initiative to reduce the environmental impact of the transport sector. The vast majority of production is currently from first-generation feedstocks, such as rapeseed oil, and waste cooking oils. However, the increased exploitation of soybean oil and palm oil has led to vast deforestation, smog emissions and heavily impacted on biodiversity in tropical regions. One promising alternative, potentially capable of meeting future demand sustainably, are oleaginous yeasts. Despite being known about for 143 years, there has been an increasing effort in the last decade to develop a viable industrial system, with currently around 100 research papers published annually. In the academic literature, approximately 160 native yeasts have been reported to produce over 20% of their dry weight in a glyceride-rich oil. The most intensively studied oleaginous yeast have been Cutaneotrichosporon oleaginosus (20% of publications), Rhodotorula toruloides (19%) and Yarrowia lipolytica (19%). Oleaginous yeasts have been primarily grown on single saccharides (60%), hydrolysates (26%) or glycerol (19%), and mainly on the mL scale (66%). Process development and genetic modification (7%) have been applied to alter yeast performance and the lipids, towards the production of biofuels (77%), food/supplements (24%), oleochemicals (19%) or animal feed (3%). Despite over a century of research and the recent application of advanced genetic engineering techniques, the industrial production of an economically viable commodity oil substitute remains elusive. This is mainly due to the estimated high production cost, however, over the course of the twenty-first century where climate change will drastically change global food supply networks and direct governmental action will likely be levied at more destructive crops, yeast lipids offer a flexible platform for localised, sustainable lipid production. Based on data from the large majority of oleaginous yeast academic publications, this review is a guide through the history of oleaginous yeast research, an assessment of the best growth and lipid production achieved to date, the various strategies employed towards industrial production and importantly, a critical discussion about what needs to be built on this huge body of work to make producing a yeast-derived, more sustainable, glyceride oil a commercial reality. The online version contains supplementary material available at 10.1186/s12934-021-01712-1.
登录
查看更多内容
影响因子：
7.1
作者：
BANG, HO;DYERBERG, J;SINCLAIR, HM
通讯作者：
SINCLAIR, HM
影响因子：
6.3
作者：
Abeln, Felix;Hicks, Robert H.;Chuck, Christopher J.
通讯作者：
Chuck, Christopher J.
影响因子：
11.4
作者：
Angerbauer, C.;Siebenhofer, M.;Guebitz, G. M.
通讯作者：
Guebitz, G. M.
影响因子：
11.1
作者：
Arous, Fatma;Frikha, Fakher;Mechichi, Tahar
通讯作者：
Mechichi, Tahar
影响因子：
7.4
作者：
Bonatsos, Nikolaos;Marazioti, Constantina;Kookos, Ioannis K.
通讯作者：
Kookos, Ioannis K.