Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia.
Seminal bacterial composition in patients with obstructive and non-obstructive azoospermia.
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梗阻性和非梗阻性无精症患者的精液细菌组成
DOI：
10.3892/etm.2018.5778
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发表时间：
2018-03
影响因子：
2.7
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Chen H;Luo T;Chen T;Wang G
许多依赖于培养物和不依赖于培养物的研究报告称，精液中细菌种类的数量和重要性可能被低估了。本研究的目的是分析梗阻性或非梗阻性无精症患者的精液微生物组。采用高通量测序方法对健康人（C组）、梗阻性无精症患者（OA组）和非梗阻性无精症患者（NOA组）精液中提取的基因组DNA进行测序。结果显示，C（98.14％）、OA（98.26％）和NOA（90.96％）组中的大多数细菌为厚壁菌门、变形菌门、拟杆菌门和放线菌门。与C组相比，OA组和NOA组的拟杆菌门和厚壁菌门增加，而变形菌门和放线菌门数量减少。共鉴定出 398 个常见操作分类单位，其中 27 个属于乳杆菌属。此外，通过重建未观察状态分析进行的群落系统发育研究表明，无精子症患者精液中的致病物种和生物多样性的减少可能导致代谢性疾病、传染性疾病和免疫性疾病的风险增加。在本研究中，对阻塞性或非阻塞性无精子症患者的精液微生物组进行了探索，这可能有助于开发针对无精子症的新疗法及其诊断。
A number of culture-dependent and -independent studies have reported that the number and significance of bacterial species in semen may have been underestimated. The aim of the present study was to profile the seminal microbiome in patients with obstructive or non-obstructive azoospermia. A high-throughput sequencing method was adopted to sequence genomic DNA extracted from the semen of healthy people (C group), patients with obstructive azoospermia (OA group) and patients with non-obstructive azoospermia (NOA group). The results revealed that Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria species comprised the majority of bacteria in the C (98.14%), OA (98.26%) and NOA (90.96%) groups. Patients in the OA and NOA groups exhibited an increase in Bacteroidetes and Firmicutes, whereas the number of Proteobacteria and Actinobacteria were decreased compared with the C group. A total of 398 common operational taxonomic units were identified, of which 27 belonged to the genus Lactobacillus. Furthermore, Phylogenetic Investigation of Communities by Reconstruction of Unobserved States analysis indicated that the pathogenic species and reduced biodiversity in the semen of patients with azoospermia may result in an increased risk of metabolic, infectious and immune diseases. In the present study, the seminal microbiome of patients with obstructive or non-obstructive azoospermia was explored, which may be useful for developing novel treatments against azoospermia as well as for its diagnosis.
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