Structural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula.
Structural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula.
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中草药配方缓解 2 型糖尿病期间肠道微生物群的结构调节
DOI：
10.1038/ismej.2014.177
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发表时间：
2015-03
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肠道微生物群被假设在代谢疾病中起关键作用，包括2型糖尿病（T2D）。传统中药方剂葛根芩连汤（GQD）可缓解T2D。为了确定GQD是否调节T2D治疗期间肠道微生物群的组成，187名T2D患者在双盲试验中随机分配接受高剂量（HD，n=44）、中等剂量（MD，n=52）、低剂量GQD（LD，n=50）或安慰剂（n=41）12周。与安慰剂组和LD组相比，接受HD或MD治疗的患者空腹血糖（FBG）和糖化血红蛋白（HbA1c）较基线的校正平均变化显著降低。16S rRNA基因的V3区的焦磷酸测序揭示了响应于GQD处理的肠道微生物群的剂量依赖性偏差。该偏离发生在观察到T2D症状显著改善之前。Redestimation分析确定了47种GQD富集的物种水平的HbA1c类型，其中17种与FBG呈负相关，9种与HbA1c呈负相关。实时荧光定量PCR证实GQD显著富集普氏粪杆菌，且与FBG、HbA1c和餐后2 h血糖水平呈负相关，与稳态模型评估的β细胞功能呈正相关。因此，这些数据表明，肠道微生物群的结构变化是由中草药配方GQD诱导的。具体来说，GQD处理可以富集有益细菌的数量，例如粪杆菌属。总之，肠道微生物群的变化与GQD的抗糖尿病作用相关。
The gut microbiota is hypothesized to have a critical role in metabolic diseases, including type 2 diabetes (T2D). A traditional Chinese herbal formula, Gegen Qinlian Decoction (GQD), can alleviate T2D. To find out whether GQD modulates the composition of the gut microbiota during T2D treatment, 187 T2D patients were randomly allocated to receive high (HD,n=44), moderate (MD,n=52), low dose GQD (LD,n=50) or the placebo (n=41) for 12 weeks in a double-blinded trial. Patients who received the HD or MD demonstrated significant reductions in adjusted mean changes from baseline of fasting blood glucose (FBG) and glycated hemoglobin (HbA1c) compared with the placebo and LD groups. Pyrosequencing of the V3 regions of 16S rRNA genes revealed a dose-dependent deviation of gut microbiota in response to GQD treatment. This deviation occurred before significant improvement of T2D symptoms was observed. Redundancy analysis identified 47 GQD-enriched species level phylotypes, 17 of which were negatively correlated with FBG and 9 with HbA1c. Real-time quantitative PCR confirmed that GQD significantly enrichedFaecalibacterium prausnitzii, which was negatively correlated with FBG, HbA1c and 2-h postprandial blood glucose levels and positively correlated with homeostasis model assessment of β-cell function. Therefore, these data indicate that structural changes of gut microbiota are induced by Chinese herbal formula GQD. Specifically, GQD treatment may enrich the amounts of beneficial bacteria, such asFaecalibacteriumspp. In conclusion, changes in the gut microbiota are associated with the anti-diabetic effects of GQD.
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