Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells.
Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells.
复制标题
DOI：
10.1371/journal.pone.0012968
复制
发表时间：
2010-09-23
期刊：
影响因子：
3.7
通讯作者：
Han JY
中科院分区：
文献类型：
--
作者：
Choi JW;Kim S;Kim TM;Kim YM;Seo HW;Park TS;Jeong JW;Song G;Han JY
在体外长期维持禽类原始生殖细胞（PGC）具有巨大的潜力，因为它可以用来加深我们对 PGC 生物学的理解。转基因生物反应器基于 PGC 通过血流向受体性索的独特迁移，从而产生种系嵌合鸟，具有许多潜在的应用。然而，诱导鸡 PGC 增殖所必需的生长因子和信号通路尚不清楚。因此，我们进行了这项研究，以探讨生长因子的各种组合对无饲养条件下 PGC 存活和增殖的影响。我们观察到 PGC 在含有 bFGF 的培养基中增殖。随后的表征证实，培养的 PGC 保持了 PGC 特异性标记物的表达、端粒酶活性、正常的迁移活性和种系传递。我们还发现 bFGF 激活丝裂原激活蛋白激酶激酶/细胞外信号调节激酶 (MEK/ERK) 信号传导。此外，133 个转录物的表达因 bFGF 的撤除而发生可逆改变。我们的结果表明，在无饲养层培养条件下，鸡 PGC 可以在体外维持，而不发生任何分化或去分化，随后的分析表明，bFGF 是通过 MEK/ERK 信号调节下游基因实现鸡 PGC 增殖的关键因素之一，这些下游基因可能对 PGC 增殖和存活很重要。
Long-term maintenance of avian primordial germ cells (PGCs) in vitro has tremendous potential because it can be used to deepen our understanding of the biology of PGCs. A transgenic bioreactor based on the unique migration of PGCs toward the recipients' sex cord via the bloodstream and thereby creating a germline chimeric bird has many potential applications. However, the growth factors and the signaling pathway essential for inducing proliferation of chicken PGCs are unknown. Therefore, we conducted this study to investigate the effects of various combinations of growth factors on the survival and proliferation of PGCs under feeder-free conditions. We observed proliferation of PGCs in media containing bFGF. Subsequent characterization confirmed that the cultured PGCs maintained expression of PGC-specific markers, telomerase activity, normal migrational activity, and germline transmission. We also found that bFGF activates the mitogen-activated protein kinase kinase/extracellular-signal regulated kinase (MEK/ERK) signaling. Also, the expression of 133 transcripts was reversibly altered by bFGF withdrawal. Our results demonstrate that chicken PGCs can be maintained in vitro without any differentiation or dedifferentiation in feeder free culture conditions, and subsequent analysis revealed that bFGF is one of the key factors that enable proliferation of chicken PGCs via MEK/ERK signaling regulating downstream genes that may be important for PGC proliferation and survival.
登录
查看更多内容
影响因子：
5.2
作者：
Jung, JS;Kim, DK;Han, JY
通讯作者：
Han, JY
影响因子：
4.8
作者：
Ali, Nazim;Knaeuper, Vera
通讯作者：
Knaeuper, Vera
影响因子：
2.5
作者：
ANDO, Y;FUJIMOTO, T
通讯作者：
FUJIMOTO, T
影响因子：
4
作者：
Fan, Lianchun;Moon, Jesung;Collodi, Paul
通讯作者：
Collodi, Paul
影响因子：
2.5
作者：
Kim, JN;Kim, MA;Han, JY
通讯作者：
Han, JY