Relative Initial Weight Is Associated with Improved Survival without Altering Tumor Latency in a Translational Rat Model of Diethylnitrosamine-Induced Hepatocellular Carcinoma and Transarterial Embolization.
Relative Initial Weight Is Associated with Improved Survival without Altering Tumor Latency in a Translational Rat Model of Diethylnitrosamine-Induced Hepatocellular Carcinoma and Transarterial Embolization.
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DOI：
10.1016/j.jvir.2017.03.037
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发表时间：
2017-07
期刊：
影响因子：
--
通讯作者：
Gade TP
中科院分区：
文献类型：
--
作者：
Kiefer RM;Hunt SJ;Pulido S;Pickup S;Furth EE;Soulen MC;Nadolski GJ;Gade TP
在二乙基亚硝胺（DEN）诱导的HCC大鼠模型中，检验初始体重增加与生存期相对改善和纤维化减少相关的假设，以及与颈动脉入路相比，经股动脉入路的TAE（TAE）减少手术时间的假设。雄性Wistar大鼠138只，自由饮水，连续12周。大鼠每周接受T2-MRI以检测HCC。在形成≥ 5 mm肿瘤后，在荧光镜引导下通过颈动脉或股动脉插管对大鼠进行选择性TAE。基于初始体重将大鼠回顾性地分为3组（<300 g、300- 400 g、> 400 g），用于分析存活、肿瘤潜伏期和纤维化。比较穿刺部位与手术成功率、死亡率和时间的相关结果。肿瘤潜伏期与体重组无显著差异（p=0.310）。体重<300 g的大鼠的存活时间比两个较大组都短（平均值= 88 d vs. 108 d，p<0.0001），纤维化更严重（<300 g中位数=4.0，300- 400 g中位数=1.5，> 400 g中位数=1.0; p=0.015）。基于入路部位的围手术期死亡率无显著差异;然而，经股动脉入路的手术时间更短（平均值=71+/-23分钟vs. 127+/-24分钟; p<0.0001）。对于DEN诱导的HCC大鼠，更大的初始体重导致存活率提高，而不延长肿瘤潜伏期，并且与不太严重的纤维化相关。在该模型中，经股动脉入路行TAE缩短了手术时间。这些修改提供了一个翻译的HCC和TAE的动物模型，可能适合于短期生存研究。
To test the hypotheses that greater initial body weight is associated with a relative improvement in survival with diminished fibrosis in a diethylnitrosamine (DEN)-induced rat model of HCC and that TAE (TAE) via femoral artery access decreases procedure times compared to carotid artery access. 138 male Wistar rats were administered 0.01% DEN in water ad libitum for 12 weeks. Rats received weekly T2-MRI to detect HCC. Upon development of ≥5mm tumors, rats underwent selective TAE via carotid or femoral artery catheterization under fluoroscopic guidance. Rats were retrospectively categorized into 3 groups (<300g, 300-400g, >400g) based on initial weight for analyses of survival, tumor latency, and fibrosis. Site of access was compared relative to procedural outcomes of success, mortality, and time. No significant differences in tumor latency were related to weight group (p=0.310). Rats weighing <300g had shorter survival time than both larger groups (mean=88d vs. 108d, p<0.0001) and more severe fibrosis (<300g median=4.0, 300-400g median=1.5, >400g median=1.0; p=0.015). No significant difference was found in peri-procedural mortality based on access site; however, procedure times were shorter via a femoral approach (mean=71+/-23min vs. 127+/-24min; p<0.0001). Greater initial body weight resulted in improved survival without prolonging tumor latency for rats with DEN-induced HCCs and was associated with less severe fibrosis. A femoral approach for TAE in this model resulted in decreased procedure time. These modifications provide a translational animal model of HCC and TAE that may be suited for short-term survival studies.
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影响因子：
2.9
作者：
Thompson, Scott M.;Callstrom, Matthew R.;Knudsen, Bruce;Anderson, Jill L.;Carter, Rickey E.;Grande, Joseph P.;Roberts, Lewis R.;Woodrum, David A.
通讯作者：
Woodrum, David A.
影响因子：
13.5
作者：
Llovet, Josep M.;Bruix, Jordi
通讯作者：
Bruix, Jordi
DOI：
10.1016/j.jvir.2008.12.408
发表时间：
2009-03-01
影响因子：
2.9
作者：
Ju, Shenghong;McLennan, Gordon;Jayaram, Hiremagalur N.
通讯作者：
Jayaram, Hiremagalur N.
影响因子：
13.5
作者：
Bedossa, P;Poynard, T
通讯作者：
Poynard, T
影响因子：
1.5
作者：
Williams, GM;Iatropoulos, MJ;Jeffrey, AM
通讯作者：
Jeffrey, AM