Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions.
Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions.
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PVY 感染下烟草 miRNA 和 mRNA 表达谱的综合分析提供了对烟草-PVY 相互作用的深入了解
DOI:
10.1038/s41598-017-05155-w
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发表时间:
2017-07-07
影响因子:
4.6
通讯作者:
Shamsi IH
中科院分区:
文献类型:
--
作者:
Guo Y;Jia MA;Yang Y;Zhan L;Cheng X;Cai J;Zhang J;Yang J;Liu T;Fu Q;Zhao J;Shamsi IH
Potato virus Y(PVY) is a globally and economically important pathogen of potato, tobacco, tomato and other staple crops and caused significant yield losses and reductions in quality.To explore the molecular PVY-host interactions, we analysed changes in the miRNA and mRNA profiles of tobacco in response to PVY infection. A total of 81 differentially expressed miRNAs belonging to 29 families and 8133 mRNAs were identified. The Gene Ontology (GO) enrichment analyses showed that genes encoding the DNA/RNA binding, catalytic activity and signalling molecules were all significantly enriched. Moreover, 88 miRNA-mRNA interaction pairs were identified through a combined analysis of the two datasets. We also found evidence showing that the virus-derived siRNAs (vsiRNAs) from the PVY genome target tobacco translationally controlled tumor protein (NtTCTP) mRNA and mediate plant resistance to PVY. Together, our findings revealed that both miRNA and mRNA expression patterns can be changed in response to PVY infection and novel vsiRNA-plant interactions that may regulate plant resistance to PVY. Both provide fresh insights into the virus-plant interactions.
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