Integrated transcriptome-proteome analyses of human stem cells reveal source-dependent differences in their regenerative signature.
Integrated transcriptome-proteome analyses of human stem cells reveal source-dependent differences in their regenerative signature.
复制标题
DOI：
10.1016/j.stemcr.2022.11.006
复制
发表时间：
2023-01-10
影响因子：
5.9
通讯作者：
Thakor, Avnesh S.
中科院分区：
文献类型：
--
作者：
Ganguly, Abantika;Swaminathan, Ganesh;Garcia-Marques, Fernando;Regmi, Shobha;Yarani, Reza;Primavera, Rosita;Chetty, Shashank;Bermudez, Abel;Pitteri, Sharon J.;Thakor, Avnesh S.
间充质干细胞（MSC）作为一种有效的再生细胞疗法越来越受到重视。然而，确保在临床人群中的一致和可靠的效果已被证明是具有挑战性的。在某种程度上，这可以归因于MSC的内在分子和再生特征的异质性，这取决于它们的来源。目前的工作使用集成组学为基础的分析，在不同的功能水平，比较的抗炎，免疫调节，和血管生成之间的MSC从新生儿（脐带MSC [UC-MSC]）和成人（脂肪组织MSC [AD-MSC]，和骨髓MSC [BM-MSC]）来源的性能。使用多参数分析，我们确定UC-MSC促进更强大的宿主先天免疫应答;相比之下，成体MSC似乎促进细胞外基质（ECM）的重塑，具有更强的血管生成级联激活。这些数据应该有助于促进来源特异性MSC的标准化，使得它们的再生特征可以自信地用于靶向特定的疾病过程。来自不同来源的MSC表现出分子和功能异质性基于Omics的功能分析定义了来源特异性再生特征UC-MSC促进强有力的立即抗炎反应成年MSC促进ECM重塑并促进血管生成在最近发表的研究中，Thakor et al.使用整合转录组蛋白质组和分泌组分析，以确定来自不同组织来源的人间充质干细胞（MSC）之间的分子和功能“签名”的差异。这些数据将有助于促进正确的MSC选择用于不同疾病的组织再生。
Mesenchymal stem cells (MSCs) are gaining increasing prominence as an effective regenerative cellular therapy. However, ensuring consistent and reliable effects across clinical populations has proved to be challenging. In part, this can be attributed to heterogeneity in the intrinsic molecular and regenerative signature of MSCs, which is dependent on their source of origin. The present work uses integrated omics-based profiling, at different functional levels, to compare the anti-inflammatory, immunomodulatory, and angiogenic properties between MSCs from neonatal (umbilical cord MSC [UC-MSC]) and adult (adipose tissue MSC [AD-MSC], and bone marrow MSC [BM-MSC]) sources. Using multi-parametric analyses, we identified that UC-MSCs promote a more robust host innate immune response; in contrast, adult-MSCs appear to facilitate remodeling of the extracellular matrix (ECM) with stronger activation of angiogenic cascades. These data should help facilitate the standardization of source-specific MSCs, such that their regenerative signatures can be confidently used to target specific disease processes. MSCs from different sources exhibit molecular and functional heterogeneity Omics-based functional analysis defines source-specific regenerative signature UC-MSCs promote robust immediate anti-inflammatory responses Adult-MSCs facilitate ECM remodeling and promote angiogenesis In a recently published study, Thakor et al. used integrative transcriptomic-proteomic and secretome analyses to identify differences in the molecular and functional “signature” between human mesenchymal stem cells (MSCs) derived from different tissue sources. These data will help to facilitate the correct MSC choice for tissue regeneration in different diseases.
登录
查看更多内容
影响因子：
4.6
作者：
Billing AM;Ben Hamidane H;Dib SS;Cotton RJ;Bhagwat AM;Kumar P;Hayat S;Yousri NA;Goswami N;Suhre K;Rafii A;Graumann J
通讯作者：
Graumann J
影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
14.9
作者：
Gillespie M;Jassal B;Stephan R;Milacic M;Rothfels K;Senff-Ribeiro A;Griss J;Sevilla C;Matthews L;Gong C;Deng C;Varusai T;Ragueneau E;Haider Y;May B;Shamovsky V;Weiser J;Brunson T;Sanati N;Beckman L;Shao X;Fabregat A;Sidiropoulos K;Murillo J;Viteri G;Cook J;Shorser S;Bader G;Demir E;Sander C;Haw R;Wu G;Stein L;Hermjakob H;D'Eustachio P
通讯作者：
D'Eustachio P
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
5.5
作者：
Cao, GY;O'Brien, CD;DeLisser, HM
通讯作者：
DeLisser, HM