Evaluation of genetic variability among "Early Mature" Juglans regia using microsatellite markers and morphological traits.
Evaluation of genetic variability among "Early Mature" Juglans regia using microsatellite markers and morphological traits.
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作者：
Ebrahimi A;Zarei A;Zamani Fardadonbeh M;Lawson S
限制幼年期和减小树的大小是育种者改善大多数水果作物的两个主要挑战。早熟和矮化品种已被报道用于许多果树品种。实生核桃中存在“早熟”和低活力的基因型。利用9个微卫星标记对早熟核桃种质资源进行遗传多样性分析，并与正常生长核桃种质资源进行比较。还在“早熟”样品中测量了六个成熟相关特征。表型性状和多样性指数表明，“早熟”幼苗的遗传多样性水平较高，表明个体间的分化较高。苗高是表现型多样性最大的性状，在早熟种质的聚类中起着重要作用。与“正常生长”组相比，“早熟”型具有较高的等位基因数、有效等位基因数和香农指数。这两种类型的核桃具有不同的等位基因，其中超过一半的等位基因特异于特定的群体。“早熟”和“正常生长”核桃分别有27和17个私人等位基因。用不同方法分离“早熟”和“正常生长”样品。“早熟”核桃中存在中度到高度的遗传多样性，与“正常生长”核桃存在高度的遗传分化，表明“早熟”核桃与“正常生长”核桃样品更多样化和不同。此外，我们的研究结果表明，SSR标记是有用的区分“早熟”和“正常生长”的核桃。许多已鉴定的基因座在育种程序中具有在萌芽阶段鉴定“早熟”核桃的潜力。
Limiting the juvenile phase and reducing tree size are the two main challenges for breeders to improve most fruit crops. Early maturation and dwarf cultivars have been reported for many fruit species. “Early mature” and low vigor walnut genotypes were found among seedlings of Persian walnut. Nine microsatellite markers were used to evaluate genetic diversity among “Early Mature” Persian walnut accessions and provide a comparison with “normal growth” accessions. Six maturation related characteristics were also measured in “Early Mature” samples. Phenotypic traits and diversity indices showed relatively high levels of genetic diversity in “Early Mature” seedlings and indicated high differentiation between individuals. Seedling height, the most diverse phenotypic trait, has an important role in the clustering of “Early Mature” accessions. The “Early Mature” type had higher number of alleles, number of effective allele, and Shannon index compared to the “Normal Growth” group. The two types of studied walnuts had different alleles, with more than half of produced alleles specific to a specific group. “Early Mature” and “Normal Growth” walnuts had 27 and 17 private alleles, respectively. Grouping with different methods separated “Early Mature” and “Normal Growth” samples entirely. The presence of moderate to high genetic diversity in “Early Mature” walnuts and high genetic differentiation with “Normal Growth” walnuts, indicated that “Early Mature” walnuts were more diverse and distinct from “Normal Growth” samples. Moreover, our results showed SSR markers were useful for differentiating between “Early Mature” and “Normal Growth” walnuts. A number of identified loci have potential in breeding programs for identification of “Early Mature” walnuts at the germination phase.
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影响因子：
5.9
作者：
Dierig, DA;Ray, DT;Lorenz, G
通讯作者：
Lorenz, G
影响因子：
2.4
作者：
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DOI：
10.1080/14620316.2009.11512509
发表时间：
2009-03-01
影响因子：
1.9
作者：
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作者：
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通讯作者：
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影响因子：
4.3
作者：
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