Multivalent interaction-based carbohydrate biosensors for signal amplification.
Multivalent interaction-based carbohydrate biosensors for signal amplification.
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DOI：
10.1016/j.bios.2010.08.025
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发表时间：
2010-11-15
影响因子：
12.6
通讯作者：
Zeng X
中科院分区：
文献类型：
--
作者：
Wang Y;Chalagalla S;Li T;Sun XL;Zhao W;Wang PG;Zeng X
研究了固定在石英晶体微天平（QCM）传感器表面的硼酸与糖修饰的Au -纳米粒子（AuNP）之间的多价相互作用，并将其用于开发灵敏的糖生物传感器。简而言之，作为多价碳水化合物受体的含硼酸的聚合物（含硼聚合物）通过异硫键形成定向固定在半胱胺涂覆的电极上。将碳水化合物与AuNP缀合以产生多价碳水化合物部分来放大响应信号。因此，碳水化合物缀合的AuNP与硼聚合物表面的结合是多价的，这可以同时增加结合亲和力和特异性。我们系统地研究了五个碳水化合物共轭金纳米粒子和硼聚合物之间的结合。研究表明，缔合常数（Ka）的顺序为岩藻糖<葡萄糖<甘露糖<半乳糖<麦芽糖。甘露糖缀合的AuNP和硼聚合物识别元件在23 μM至3.83 mM范围内观察到线性响应，碳水化合物分析物的检测下限为1.5 μM。此外，碳水化合物和硼酸之间的多价结合是可逆的，并且允许通过使用IM乙酸再生硼聚合物表面，以便顺序地捕获和释放碳水化合物分析物。
Multivalent interaction between boronic acids immobilized on Quartz Crystal Microbalance (QCM) sensor surface and the carbohydrates modified Au - nanoparticle (AuNP) has been demonstrated for the development of a sensitive carbohydrate biosensor. Briefly, a boronic acid - containing polymer (boropolymer) as multivalent carbohydrate receptor was oriented immobilized on the cysteamine coated electrode through isourea bond formation. Carbohydrates were conjugated to AuNPs to generate a multivalent carbohydrates moiety to amplify the response signal. Thus, the binding of the carbohydrate conjugated AuNPs to the boropolymer surface are multivalent which could simultaneously increase the binding affinity and specificity. We systematically studied the binding between five carbohydrate conjugated AuNPs and the boropolymer. Our studies show that the associate constant (Ka) was in the order of fucose < glucose < mannose < galactose < maltose. A linear response in the range from 23 µM to 3.83 mM was observed for mannose conjugated AuNPs and the boropolymer recognition elements, with the lower detection limit of 1.5 µM for the carbohydrate analytes. Furthermore, the multivalent binding between carbohydrates and boronic acids are reversible and allow the regeneration of boropolymer surface by using 1M acetic acid so as to sequentially capture and release the carbohydrate analytes.
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影响因子：
7.4
作者：
Shen, ZH;Mernaugh, RL;Zeng, XQ
通讯作者：
Zeng, XQ
影响因子：
3.6
作者：
LORAND, JP;EDWARDS, JO
通讯作者：
EDWARDS, JO
影响因子：
4.3
作者：
Nagase, T;Nakata, E;Hamachi, I
通讯作者：
Hamachi, I
影响因子：
2.9
作者：
Kobayashi, Y;Nakamura, H;Kawagishi, H
通讯作者：
Kawagishi, H
影响因子：
12.6
作者：
Liu Y;Liu Y;Mernaugh RL;Zeng X
通讯作者：
Zeng X