Slicing the Embryonic Chicken Auditory Brainstem to Evaluate Tonotopic Gradients and Microcircuits.
Slicing the Embryonic Chicken Auditory Brainstem to Evaluate Tonotopic Gradients and Microcircuits.
复制标题
鸡胚听性脑干切片以评估音调分布和微电路。
DOI：
10.3791/63476
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发表时间：
2022-07-12
期刊：
影响因子：
--
通讯作者：
Sanchez JT
中科院分区：
文献类型：
--
作者：
Mohan S;Roy A;Ordiway G;Sanchez JT
鸡胚是一种被广泛接受的研究听觉脑干的动物模型，它由高度专门化的微电路和沿张力轴（即频率轴）定向的神经元拓扑结构组成。声位轴允许在喙侧-内侧平面分离高频声音编码，在尾侧区域分离低频声音编码。传统上，胚胎组织的冠状脑干切片允许研究相对个体的等频层。虽然足以研究与个体等频区有关的解剖学和生理学问题，但对听觉脑干大区域的张力异位变异及其发展的研究在一定程度上是有限的。本协议报告了鸡胚胎的脑干切片技术，该技术包含了下听觉脑干中较大的频率梯度区域。利用不同的切片方法对鸡听脑干组织进行电生理和解剖实验，在一个脑干切片中，与冠状面切片相比，更大的张力梯度和发育轨迹得到了更好的保存。多种切片技术可以改进对听觉脑干微回路的各种解剖、生物物理和张力变化特性的研究。在这里，我们提出了一种获得鸡胚非冠状听觉脑干切片的方案，用于研究一个脑干切片的张力特性和发育轨迹。这些切片包括矢状面、水平面和水平/横切面，在单个切片平面内包含比传统冠状面更大的肌瘤区域。
The chicken embryo is a widely accepted animal model to study the auditory brainstem, composed of highly specialized microcircuitry and neuronal topology differentially oriented along a tonotopic (i.e., frequency) axis. The tonotopic axis permits the segregated encoding of high-frequency sounds in the rostral-medial plane and low-frequency encoding in caudo-lateral regions. Traditionally, coronal brainstem slices of embryonic tissue permit the study of relative individual iso-frequency lamina. Although sufficient to investigate anatomical and physiological questions pertaining to individual iso-frequency regions, the study of tonotopic variation and its development across larger auditory brainstem areas is somewhat limited. This protocol reports brainstem slicing techniques from chicken embryos that encompass larger gradients of frequency regions in the lower auditory brainstem. The utilization of different slicing methods for chicken auditory brainstem tissue permits electrophysiological and anatomical experiments within one brainstem slice, where larger gradients of tonotopic properties and developmental trajectories are better preserved than coronal sections. Multiple slicing techniques allow for improved investigation of the diverse anatomical, biophysical, and tonotopic properties of auditory brainstem microcircuits. Here we present a protocol for obtaining non-coronal auditory brainstem slices of the chicken embryo for the investigation of tonotopic properties and developmental trajectories within one brainstem slice. These slices include sagittal, horizontal, and horizontal/transverse sections encompassing larger tonotopic regions within an individual slice plane that the traditional coronal sections.
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影响因子：
2.8
作者：
Passetto, Maria F.;Britto, Luiz R. G.;Toledo, Claudio A. B.
通讯作者：
Toledo, Claudio A. B.
影响因子：
2.5
作者：
RUBEL, EW;PARKS, TN
通讯作者：
PARKS, TN
影响因子：
2.5
作者：
Fukui, Iwao;Sato, Tatsuo;Ohmori, Harunori
通讯作者：
Ohmori, Harunori
DOI：
10.1007/978-3-319-21530-3_4
发表时间：
2017-01-01
期刊：
AUDITORY DEVELOPMENT AND PLASTICITY: IN HONOR OF EDWIN W RUBEL
影响因子：
--
作者：
Sanchez, Jason Tait;Lu, Yong
通讯作者：
Lu, Yong
影响因子：
5.3
作者：
Wang, Xiaoyu;Zorio, Diego A. R.;Wang, Yuan
通讯作者：
Wang, Yuan