Interspecific small molecule interactions between clinical isolates of Pseudomonas aeruginosa and Staphylococcus aureus from adult cystic fibrosis patients.
Interspecific small molecule interactions between clinical isolates of Pseudomonas aeruginosa and Staphylococcus aureus from adult cystic fibrosis patients.
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DOI：
10.1371/journal.pone.0086705
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Malouin F
中科院分区：
文献类型：
--
作者：
Fugère A;Lalonde Séguin D;Mitchell G;Déziel E;Dekimpe V;Cantin AM;Frost E;Malouin F
铜绿假单胞菌和金黄色葡萄球菌是囊性纤维化（CF）患者气道感染中最常见的病原体。我们研究了这些病原体是如何共存和相互作用的。从成年CF患者中分离出两种菌株。当将63株铜绿假单胞菌分离株的培养上清液添加到对照金黄色葡萄球菌培养物中时，引发了广泛的生物膜刺激活性。金黄色葡萄球菌形成生物膜的程度与铜绿假单胞菌产生的2-烷基-4-(1H)-喹诺酮（AQs）假单胞菌喹诺酮信号（PQS）和2-庚基-4-羟基喹啉n -氧化物（HQNO）水平呈正相关。与亲本菌株PA14相比，PQS和HQNO缺失的铜绿假单胞菌等基因突变体的上清液对金黄色葡萄球菌生物膜形成的刺激明显减少。当研究铜绿假单胞菌和金黄色葡萄球菌从显示两种病原体的患者中提取的共分离对时，与使用对照金黄色葡萄球菌菌株观察到的相比，铜绿假单胞菌上清液刺激金黄色葡萄球菌对应物产生的生物膜较少。因此，一些铜绿假单胞菌分离株产生低水平的外产物，而且一些临床金黄色葡萄球菌分离株没有受到它们的共分离株或PA14的刺激，尽管产生了足够的HQNO。这表明CF肺的定植促进了某种类型的菌株选择，或者共存需要病原体中的一方或双方的特定适应。结果提供了CF中细菌相互作用的见解。
Pseudomonas aeruginosa and Staphylococcus aureus are the most prevalent pathogens in airway infections of cystic fibrosis (CF) patients. We studied how these pathogens coexist and interact with each other. Clinical isolates of both species were retrieved from adult CF patients. Culture supernatants from 63 P. aeruginosa isolates triggered a wide range of biofilm-stimulatory activities when added to the culture of a control S. aureus strain. The extent of biofilm formation by S. aureus was positively correlated to the levels of the 2-alkyl-4-(1H)-quinolones (AQs) Pseudomonas Quinolone Signal (PQS) and 2-heptyl-4-hydroxy quinoline N-oxide (HQNO) produced by the P. aeruginosa isolates. Supernatants from P. aeruginosa isogenic mutants deficient in PQS and HQNO production stimulated significantly less biofilm formation by S. aureus than that seen with the parental strain PA14. When studying co-isolated pairs of P. aeruginosa and S. aureus retrieved from patients showing both pathogens, P. aeruginosa supernatants stimulated less biofilm production by the S. aureus counterparts compared to that observed using the control S. aureus strain. Accordingly, some P. aeruginosa isolates produced low levels of exoproducts and also some of the clinical S. aureus isolates were not stimulated by their co-isolates or by PA14 despite adequate production of HQNO. This suggests that colonization of the CF lungs promotes some type of strain selection, or that co-existence requires specific adaptations by either or both pathogens. Results provide insights on bacterial interactions in CF.
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