The fluorescent protein palette: tools for cellular imaging.

The fluorescent protein palette: tools for cellular imaging.
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DOI:
10.1039/b901966a
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发表时间:
2009-10
影响因子:
46.2
通讯作者:
Davidson MW
Davidson MW
中科院分区:
化学1区
文献类型:
--
作者:
Day RN;Davidson MW

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这篇批判性评论概述了不断扩大的荧光蛋白 (FP) 家族,这些蛋白已成为细胞生物学和生理学研究的重要工具。在这里,我们描述了基因编码荧光标记的特征,这些荧光标记现在跨越了从深蓝色到深红色的可见光谱。我们确定了一些具有不寻常特征的新型 FP,这些特征使它们成为细胞内蛋白质动态行为的有用报告者,并描述了有多少种不同的光学方法可以与 FP 相结合,以在生命系统中提供定量测量。 “如果用 Pulmo marinus 摩擦木头,它就会呈现出着火的样子;事实上,经过如此处理的手杖会像火炬一样照亮道路”(老普林尼从约翰博斯托克翻译,1855)。
This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spectrum from deep blue to deep red. We identify some of the novel FPs that have unusual characteristics that make them useful reporters of the dynamic behaviors of proteins inside cells, and describe how many different optical methods can be combined with the FPs to provide quantitative measurements in living systems. “If wood is rubbed with the Pulmo marinus, it will have all the appearance of being on fire; so much so, indeed, that a walking-stick, thus treated, will light the way like a torch” (translation of Pliny the Elder from John Bostock, 1855).
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