Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae)
Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae)
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杀菌剂减少了类酵母共生菌的数量和白背飞虱 Sogatellafurcifera（同翅目：飞虱科）的存活率。
DOI：
10.3390/insects11040209
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发表时间：
2020-03
期刊：
影响因子：
3
通讯作者：
Lin Huafeng
中科院分区：
文献类型：
--
作者：
Pang Kun;Dong Shengzhang;Hao Peiying;Chen Tongtong;Wang Xinlong;Yu Xiaoping;Lin Huafeng
白背飞虱（Sogatellafurcifera）是东南亚地区危害水稻最严重的害虫之一。白背飞虱的脂肪体含有胞内酵母样共生体（YLS）。YLS通过经卵巢感染垂直传播给白背飞虱后代。YLS在白背飞虱的生活史中起着重要的作用。尽管已经提出了基于抑制YLS的防治褐飞虱的新策略，但YLS在褐飞虱和小褐飞虱中的多样性和功能已被广泛研究，而在白飞虱中却没有。在这里，我们使用变性梯度凝胶电泳，确定了12个独特的真菌序列中的白背飞虱YLS，其中5个代表未培养的真菌。然后，我们用用不同杀菌剂[70%丙森锌粉剂（WP）（PR），70%霜霉威盐酸盐水溶液（AS）（PH），25%嘧菌酯和50%戊唑醇水分散粒剂（WG）（TT），40%嘧霉胺悬浮剂（SC）（PY），和50%异菌脲SC（IP）]对YLS丰度和白背飞虱存活率的影响。无论是YLS丰度和成年白背飞虱存活率显着下降后，喂养杀菌剂处理的水稻植株，暴露于50%的IP导致最强的减少。暴露于50%IP后，两种Sf-YLS物种（子囊菌共生体和类CLA共生体）的丰度显著降低。子囊菌共生菌（最丰富的YLS物种）的计数也被测试的其他杀真菌剂抑制。因此，50%IP是最有效的杀菌剂，在受控条件下降低YLS丰度和白背飞虱存活率，表明其潜在的用于控制白背飞虱。
The white-backed planthopper (WBPH) Sogatella furcifera is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an important role in the WBPH life cycle. YLS diversity and function have been extensively studied in the brown planthopper (BPH) and small brown planthopper but not in WBPH, even though a novel strategy for controlling the BPH based on suppressing YLS has been proposed. Here, using denaturing gradient gel electrophoresis, we identified 12 unique fungal sequences among YLS of WBPH, and five of them represented uncultured fungi. We then fed WBPH with rice plants treated with different fungicides [70% propineb wettable powder (WP) (PR), 70% propamocarb hydrochloride aqueous solution (AS) (PH), 25% trifloxystrobin and 50% tebuconazole water-dispersible granules (WG) (TT), 40% pyrimethanil suspension concentrate (SC) (PY), and 50% iprodione SC (IP)] and evaluated their effects on YLS abundance and WBPH survival rate. Both YLS abundance and adult WBPH survival rate were significantly decreased upon feeding fungicide-treated rice plants, and exposure to 50% IP resulted in the strongest reduction. The abundance of two Sf-YLS species (Ascomycetes symbiotes and Cla-like symbiotes) was significantly reduced upon exposure to 50% IP. The counts of Ascomycetes symbiotes, the most abundant YLS species, were also suppressed by the other fungicides tested. In conclusion, 50% IP was the most effective fungicide, reducing YLS abundance and WBPH survival rate under controlled conditions, suggesting its potential use to control WBPH.
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影响因子：
3.6
作者：
Kobiałka M;Michalik A;Walczak M;Szklarzewicz T
通讯作者：
Szklarzewicz T
影响因子：
3.4
作者：
H. Noda;N. Kawahara
通讯作者：
H. Noda;N. Kawahara
DOI：
10.1653/024.096.0332
发表时间：
2013-10
期刊：
--
影响因子：
--
作者：
Jianya Su;Zhiwei Wang;Kai Zhang;Xiangrui Tian;Yanqiong Yin;Xueqing Zhao;A. Shen;Congfen Gao
通讯作者：
Jianya Su;Zhiwei Wang;Kai Zhang;Xiangrui Tian;Yanqiong Yin;Xueqing Zhao;A. Shen;Congfen Gao
DOI：
10.1073/pnas.1803245115
发表时间：
2018-06-26
影响因子：
11.1
作者：
Matsuura Y;Moriyama M;Łukasik P;Vanderpool D;Tanahashi M;Meng XY;McCutcheon JP;Fukatsu T
通讯作者：
Fukatsu T
白背飞虱分泌的水状唾液的蛋白质组学分析
影响因子：
3.7
作者：
Miao YT;Deng Y;Jia HK;Liu YD;Hou ML
通讯作者：
Hou ML