Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera.

Proteomic analysis of watery saliva secreted by white-backed planthopper, Sogatella furcifera.
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白背飞虱分泌的水状唾液的蛋白质组学分析

DOI:
10.1371/journal.pone.0193831
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发表时间:
2018
期刊:
影响因子:
3.7
通讯作者:
Hou ML
Hou ML
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Miao YT;Deng Y;Jia HK;Liu YD;Hou ML

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白背飞虱,Sogatella furcifera,是一种韧皮部汁液捕食者,在进食过程中分泌水状和凝胶状唾液。在本研究中,我们采用霰弹枪LC-MS/MS分析结合转录组学分析,鉴定了S. furcifera水样唾液中的主要蛋白。共鉴定出161种蛋白质,分为8类功能,包括酶、转运蛋白、钙离子结合蛋白、唾液鞘蛋白、细胞骨架蛋白、DNA-、RNA-、蛋白质结合或调节蛋白、其他非酶蛋白和未知蛋白。采用实时荧光定量pcr分析11种分泌蛋白的基因表达谱。我们检测到黏液样蛋白在唾液腺中具有独特的表达水平,很可能是参与植物防御调节的候选效应物。本研究鉴定了水样真菌的唾液成分,并提供了可能在真菌与水稻相互作用中起作用的蛋白质序列。
The white-backed planthopper, Sogatella furcifera, is a phloem sap feeder that secretes watery and gelling saliva during feeding. In this study, we identified the major proteins in watery saliva of S. furcifera by shotgun LC-MS/MS analysis combined with transcriptomic analysis. A total of 161 proteins were identified, which were divided into 8 function categories, including enzymes, transporter, calcium ion binding protein, salivary sheath protein, cytoskeleton protein, DNA-, RNA-, and protein-binding or regulating proteins, other non-enzyme proteins and unknown proteins. Gene expression pattern of 11 secretory proteins were analyzed by real time quantitative-PCR. We detected the mucin-like protein, which had a unique expression level in salivary gland, most likely to be a candidate effector involved in regulation of plant defense. This study identified the watery saliva component of S. furcifera and it provided a list of proteins which may play a role in interaction between S. furcifera and rice.
DOI: 10.1371/journal.pone.0123671
发表时间: 2015
期刊: PloS one
影响因子: 3.7
作者:
Hattori M;Komatsu S;Noda H;Matsumoto Y
通讯作者: Matsumoto Y
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影响因子: 2.3
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