Dab2, Megalin, Cubilin and Amnionless Receptor Complex Might Mediate Intestinal Endocytosis in the Suckling Rat
Dab2, Megalin, Cubilin and Amnionless Receptor Complex Might Mediate Intestinal Endocytosis in the Suckling Rat
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Dab2、Megalin、Cubilin 和无羊膜受体复合物可能介导乳鼠的肠内吞作用
DOI：
10.1002/jcb.24685
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发表时间：
2014
影响因子：
4
通讯作者：
A. Ilundain
中科院分区：
文献类型：
--
作者：
M. Vázquez;M. Palomo;P. García;I. Sánchez;M. J. Peral;M. L. Calonge;A. Ilundain
我们以前提出Dab 2参与了乳大分子在大鼠小肠的内吞。在这里，我们调查的受体，可能介导这种内吞作用，通过研究的影响，年龄和饮食的megalin，VLDLR，ApoER 2的表达，以及年龄的表达cubilin和cubonless。在megalin、VLDLR和ApoER 2中，只有megalin的表达模式类似于先前报道的Dab 2。因此，巨蛋白和Dab 2的mRNA和蛋白质水平在乳鼠肠道中较高，主要在回肠中，随年龄下调，随牛奶饮食上调。年龄和饮食均不影响ApoER 2 mRNA水平。年龄对VLDLR mRNA水平的影响取决于测试的上皮细胞，但它们被牛奶饮食下调。在乳鼠中，cubilin和cubonless的肠道表达与megalin和megalin相似，cubilin、cubonless和Dab 2共定位于微绒毛和顶端内吞器。还观察到Dab 2与ApoER 2和VLDLR在微绒毛和顶端内吞装置中的共定位。这是第一份显示megalin/cubilin/cubonless/Dab 2、VLDLR/Dab 2和ApoER 2/Dab 2的肠道共定位的报告。我们的结论是megalin/cubilin/cubonless/Dab 2复合物/es参与肠道过程，主要是在哺乳期，Dab 2可能作为一个适配器在肠道过程中介导的ApoER 2和VLDLR。J.细胞。115：510-522，2014.© 2013 Wiley Periodicals，Inc.
We previously proposed that Dab2 participates in the endocytosis of milk macromolecules in rat small intestine. Here we investigate the receptors that may mediate this endocytosis by studying the effects of age and diet on megalin, VLDLR, and ApoER2 expression, and that of age on the expression of cubilin and amnionless. Of megalin, VLDLR and ApoER2, only the megalin expression pattern resembles that of Dab2 previously reported. Thus the mRNA and protein levels of megalin and Dab2 are high in the intestine of the suckling rat, down‐regulated by age and up‐regulated by milk diet, mainly in the ileum. Neither age nor diet affect ApoER2 mRNA levels. The effect of age on VLDLR mRNA levels depends on the epithelial cell tested but they are down‐regulated by milk diet. In the suckling rat, the intestinal expressions of both cubilin and amnionless are similar to that of megalin and megalin, cubilin, amnionless and Dab2 co‐localize at the microvilli and in the apical endocytic apparatus. Co‐localization of Dab2 with ApoER2 and VLDLR at the microvilli and in the apical endocytic apparatus is also observed. This is the first report showing intestinal co‐localization of: megalin/cubilin/amnionless/Dab2, VLDLR/Dab2 and ApoER2/Dab2. We conclude that the megalin/cubilin/amnionless/Dab2 complex/es participate in intestinal processes, mainly during the lactation period and that Dab2 may act as an adaptor in intestinal processes mediated by ApoER2 and VLDLR. J. Cell. Biochem. 115: 510–522, 2014. © 2013 Wiley Periodicals, Inc.
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DOI：
10.1016/0006-291x(91)92067-t
发表时间：
1991-12-31
影响因子：
3.1
作者：
HWANG, ES;HIRAYAMA, BA;WRIGHT, EM
通讯作者：
WRIGHT, EM
影响因子：
13.6
作者：
Amsellem, Sabine;Gburek, Jakub;Kozyraki, Renata
通讯作者：
Kozyraki, Renata
影响因子：
4
作者：
J. Wilson;J. Whitney;M. Neutra
通讯作者：
J. Wilson;J. Whitney;M. Neutra
内因子钴胺素回肠受体的分离和表征。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Seetharam,B;Alpers,DH;Allen,RH
通讯作者：
Allen,RH