Cell type specializations of the vocal-motor cortex in songbirds.
Cell type specializations of the vocal-motor cortex in songbirds.
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DOI：
10.1016/j.celrep.2023.113344
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发表时间：
2023-11-28
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
识别支持复杂行为（如习得性发声）的皮质回路中的分子特化，需要了解这些回路产生的神经解剖学背景。在鸣禽中，强健的arcopallial核（RA）为精细的声音运动控制提供下行皮质投射。利用单核转录组学和空间基因表达图谱在斑胸草雀，我们已经定义了细胞类型和分子专业化，区分RA从相邻地区参与非声乐运动和感觉处理。我们描述了RA特异性投射神经元，差异抑制亚型，和神经胶质细胞的专业化，并探讨了预测GABA能中间神经元亚型电生理RA内。一些细胞特异性标记物以性别依赖的方式在发育过程中出现。我们的交互式应用程序将细胞数据与来自基因表达大脑图谱ZEBrA的发育和空间分布数据相结合。用户可以探索发声运动神经元和支持细胞的分子特化，这些细胞可能反映了发声控制回路和精细运动技能的生理学和进化的关键适应性。Nevue等人基于snRNA-seq定义了斑胸草雀发声鲁棒弓腭核（RA）和邻近区域中的细胞类型。他们展示了RA特有的神经元和星形胶质细胞类型，以及在发声发育过程中细胞定义标记物是如何产生的。细胞类型和发育转录组学数据集可以很容易地通过交互式应用程序进行挖掘。
Identifying molecular specializations in cortical circuitry supporting complex behaviors, like learned vocalizations, requires understanding of the neuroanatomical context from which these circuits arise. In songbirds, the robust arcopallial nucleus (RA) provides descending cortical projections for fine vocal-motor control. Using single-nuclei transcriptomics and spatial gene expression mapping in zebra finches, we have defined cell types and molecular specializations that distinguish RA from adjacent regions involved in non-vocal motor and sensory processing. We describe an RA-specific projection neuron, differential inhibitory subtypes, and glia specializations and have probed predicted GABAergic interneuron subtypes electrophysiologically within RA. Several cell-specific markers arise developmentally in a sex-dependent manner. Our interactive apps integrate cellular data with developmental and spatial distribution data from the gene expression brain atlas ZEBrA. Users can explore molecular specializations of vocal-motor neurons and support cells that likely reflect adaptations key to the physiology and evolution of vocal control circuits and refined motor skills. Nevue et al. define cell types in the vocal robust arcopallial nucleus (RA) and adjacent areas of zebra finches based on snRNA-seq. They demonstrate neuronal and astrocyte types unique to RA and how cell-defining markers arise during vocal development. Cell type and developmental transcriptomics datasets can be easily mined with interactive applications.
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影响因子：
3.7
作者：
Feenders, Gesa;Liedvogel, Miriam;Rivas, Miriam;Zapka, Manuela;Horita, Haruhito;Hara, Erina;Wada, Kazuhiro;Mouritsen, Henrik;Jarvis, Erich D.
通讯作者：
Jarvis, Erich D.
影响因子：
4.2
作者：
Alcami P;Totagera S;Sohnius-Wilhelmi N;Leitner S;Grothe B;Frankl-Vilches C;Gahr M
通讯作者：
Gahr M
影响因子：
--
作者：
Carleton JB;Lovell PV;McHugh A;Marzulla T;Horback KL;Mello CV
通讯作者：
Mello CV
DOI：
10.1085/jgp.113.5.641
发表时间：
1999-05
期刊：
The Journal of general physiology
影响因子：
--
作者：
Jerng HH;Shahidullah M;Covarrubias M
通讯作者：
Covarrubias M
影响因子：
2.5
作者：
Champoux, Katharine L.;Miller, Kimberly E.;Perkel, David J.
通讯作者：
Perkel, David J.