The Bacillus subtilis and Lactic Acid Bacteria Probiotics Influences Intestinal Mucin Gene Expression, Histomorphology and Growth Performance in Broilers.
The Bacillus subtilis and Lactic Acid Bacteria Probiotics Influences Intestinal Mucin Gene Expression, Histomorphology and Growth Performance in Broilers.
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DOI：
10.5713/ajas.2012.12110
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发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
Qujeq D
中科院分区：
文献类型：
--
作者：
Aliakbarpour HR;Chamani M;Rahimi G;Sadeghi AA;Qujeq D
本研究旨在评价饲粮中添加商品单菌和多菌益生菌对肉仔鸡生长性能、肠道形态和粘蛋白基因（MUC 2）表达的影响。选用378只1日龄雄性阿利安肉仔鸡，随机分为3个试验组，试验期6 wk。用基于玉米-大豆的饮食喂养鸟，并根据添加物标记如下：对照-未补充（C），补充有基于枯草芽孢杆菌（BS）和乳酸菌（LAB）的益生菌的鸟。每个处理设6个重复，每个重复21只肉鸡。测定了对体重、采食量、饲料转化率和生物标志物如肠杯状细胞密度、绒毛长度、绒毛宽度和粘蛋白基因表达的处理效果。总采食量在对照组和饲喂益生菌组之间没有显著差异（p>0.05）。然而，在生长性能方面存在显著差异，42日龄时饲喂益生菌的鸡的最终体重高于饲喂不含益生菌的鸡（p<0.05）。与对照鸡相比，在饮食中包含基于枯草芽孢杆菌的益生菌也显著影响饲料转化率（FCR）（p<0.05）。在饲喂不同类型的益生菌补充饮食的禽类中未观察到生长性能的差异。日粮中添加乳酸菌益生菌显著提高了奶牛的杯状细胞数和绒毛长度（p<0.05）。此外，具有基于枯草芽孢杆菌的益生菌的饮食显著增加基因表达（p<0.05），与饲喂对照饮食的那些相比，饲喂具有益生菌的饮食的鸟中的肠MUC 2 mRNA更高。在BS和LAB益生菌喂养的雏鸡中，较高的生长性能可能与杯状细胞中MUC 2基因的较高表达和/或小肠的形态学改变有关。益生菌给药后粘蛋白基因的较高合成可能会积极影响肠道消化道中的细菌相互作用、肠粘膜细胞增殖以及因此有效的营养吸收。
The aim of the present study was to evaluate the effect of commercial monostrain and multistrain probiotics in diets on growth performance, intestinal morphology and mucin gene (MUC2) expression in broiler chicks. Three hundred seventy-eight 1-d-old male Arian broiler chicks were allocated in 3 experimental groups for 6 wk. The birds were fed on a corn-soybean based diet and depending on the addition were labeled as follows: control-unsupplemented (C), birds supplemented with Bacillus subtilis (BS) and lactic acid bacteria (LAB) based probiotics. Each treatment had 6 replicates of 21 broilers each. Treatment effects on body weight, feed intake, feed conversion ratio and biomarkers such as intestinal goblet cell density, villus length, villus width, and mucin gene expression were determined. Total feed intake did not differ significantly between control birds and those fed a diet with probiotics (p>0.05). However, significant differences in growth performance were found. Final body weight at 42 d of age was higher in birds fed a diet with probiotics compared to those fed a diet without probiotic (p<0.05). Inclusion of Bacillus subtilis based probiotic in the diets also significantly affected feed conversion rate (FCR) compared with control birds (p<0.05). No differences in growth performance were observed in birds fed different types of probiotic supplemented diets. Inclusion of lactic acid bacteria based probiotic in the diets significantly increased goblet cell number and villus length (p<0.05). Furthermore, diets with Bacillus subtilis based probiotics significantly increased gene expression (p<0.05), with higher intestinal MUC2 mRNA in birds fed diet with probiotics compared to those fed the control diet. In BS and LAB probiotic fed chicks, higher growth performance may be related to higher expression of the MUC2 gene in goblet cells and/or morphological change of small intestinal tract. The higher synthesis of the mucin gene after probiotic administration may positively affect bacterial interactions in the intestinal digestive tract, intestinal mucosal cell proliferation and consequently efficient nutrient absorption.
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影响因子：
5.6
作者：
Lutful Kabir SM
通讯作者：
Lutful Kabir SM
影响因子：
4.4
作者：
Chen, K. -L.;Kho, W. -L.;Hsieh, C. -W.
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4.4
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4.4
作者：
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