Long-lasting LTP requires neither repeated trains for its induction nor protein synthesis for its development.
Long-lasting LTP requires neither repeated trains for its induction nor protein synthesis for its development.
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DOI：
10.1371/journal.pone.0040823
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ris L
中科院分区：
文献类型：
--
作者：
Villers A;Godaux E;Ris L
目前关于海马CA1区触发LTP的观点有两个“教条”：(1)单次高频刺激足以触发短期LTP（1 - 3小时），而诱导长期LTP （L-LTP，超过4小时）则需要多次高频刺激。(2)L-LTP后期的发展需要合成新的蛋白质。在这项研究中，我们发现单次高频训练可以触发持续超过8小时的LTP，而不受大霉素或环己亚胺（两种蛋白质合成抑制剂）的影响。我们确定了这种L-LTP的诱导使用与通常报道的LTP诱导相同的机制：它依赖于NMDA受体和两个“核心”激酶，CaMKII和PI3K的激活。这些发现对LTP的两个“教条”提出了质疑。
Current thinking about LTP triggered in the area CA1 of hippocampal slices is ruled by two “dogmas”: (1) A single train of high-frequency stimulation is sufficient to trigger short-lasting LTP (1 – 3 h), whereas multiple trains are required to induce long-lasting LTP (L-LTP, more than 4 h). (2) The development of the late phase of L-LTP requires the synthesis of new proteins. In this study, we found that a single high-frequency train could trigger an LTP lasting more than 8 h that was not affected by either anisomycin or cycloheximide (two inhibitors of protein synthesis). We ascertained that the induction of this L-LTP made use of the same mechanisms as those usually reported to be involved in LTP induction: it was dependent on NMDA receptors and on the activation of two “core” kinases, CaMKII and PI3K. These findings call into question the two “dogmas” about LTP.
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4.7
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通讯作者：
Collingridge, GL
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作者：
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