Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein
Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein
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华支睾吸虫皮膜蛋白基因的分子克隆与鉴定
DOI：
10.1007/s00436-007-0541-8
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发表时间：
2007-05
影响因子：
2
通讯作者：
Lu, Fangli
中科院分区：
文献类型：
--
作者：
Wu, Zhongdao;Ma, Changling;Hu, Xuchu;Yu, Xinbing;Huang, Yan;Xu, Jin;Zhou, Zhenwen;Hu, Huixia;Hu, Fengyu;Chen, Xiaoxiang;Lu, Fangli
扁形线虫的被膜对于调节宿主的反应和寄生虫的生存至关重要。通过对华支睾吸虫基因文库的大规模测序，发现了一个编码新的被膜蛋白20.8 kDa(TP20.8)的基因。该基因全长755个碱基对，含有一个555个碱基的开放阅读框，编码一个20.8 kDa的等电点为4.33的蛋白质。推导的氨基酸序列与日本血吸虫sj20.8和曼氏血吸虫Sm20.8的同源性分别为40%和37%。TP20.8转录本在中华绒毛虫成虫和囊虫的cDNA文库中检测到，而在虫卵中未检测到。从大肠杆菌BL21中获得并纯化了重组中华绒毛虫TP20.8蛋白。利用特异性抗重组TP20.8蛋白血清，将TP20.8蛋白定位于中华绒毛虫外表膜。用酶联免疫吸附试验检测100例华支睾吸虫病患者、20例血吸虫病患者和30例阴性对照血清中重组抗原的特异性和敏感性。其敏感性为68%，特异性为84%。该抗原在华支睾吸虫病血清诊断中的应用价值较低。
The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A cDNA encoding a novel tegumental protein 20.8 kDa (TP20.8) was found by large-scale sequencing of a Clonorchis sinensis cDNA library. This new cDNA was 755 bp long containing an open reading frame of 555 bp, which encoded a 20.8-kDa protein with an isoelectric point of 4.33. The deduced amino acid sequence exhibits 40 and 37% identity to Schistosoma japonicum sj20.8 and Schistosoma mansoni Sm 20.8, respectively. TP20.8 transcripts were detected in the adult worm and metacercariae cDNA libraries of C. sinensis but not in the egg. Recombinant C. sinensis TP20.8 protein was produced and purified from Escherichia coli BL21. Using specific anti-recombinant TP20.8 protein sera, the TP20.8 protein was immunohistochemically localized to the outer-surface membrane of C. sinensis. The specificity and sensitivity of the recombinant antigen for serologic diagnosis was assessed by enzyme-linked immunosorbent assay using serum from 100 patients with clonorchiasis, 20 patients with schistosomiasis, and 30 negative controls. The sensitivity was 68%, and the specificity was 84%. The antigen was less useful for the serodiagnosis of clonorchiasis with IgG.
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DOI：
10.1074/jbc.272.7.4509
发表时间：
1997-02
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
H. Roder;R. Fracasso;F. Hoffman;James A. Witowsky;G. Davis;C. Pellegrino
通讯作者：
H. Roder;R. Fracasso;F. Hoffman;James A. Witowsky;G. Davis;C. Pellegrino
影响因子：
2
作者：
Wang, C. R.;Qiu, J. H.;Zhu, X. Q.
通讯作者：
Zhu, X. Q.
影响因子：
2.1
作者：
N. Zheng;Jin Xu;Zhongdao Wu;Jin-zhong Chen;Xuchu Hu;Linxia Song;Guang Yang;C. Ji;Shou-yi Chen;S. Gu;K. Ying;Xinbing Yu
通讯作者：
N. Zheng;Jin Xu;Zhongdao Wu;Jin-zhong Chen;Xuchu Hu;Linxia Song;Guang Yang;C. Ji;Shou-yi Chen;S. Gu;K. Ying;Xinbing Yu
影响因子：
--
作者：
Lee, Jae-Hyun;Rim, Han-Jong;Bak, Ung-Bok
通讯作者：
Bak, Ung-Bok
影响因子：
4
作者：
Van Hellemond, Jaap J.;Retra, Kim;Tielens, Aloysius G. M.
通讯作者：
Tielens, Aloysius G. M.