2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism.
2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism.
复制标题
DOI：
10.1038/s41421-021-00273-2
复制
发表时间：
2021-06-08
期刊：
影响因子：
33.5
通讯作者：
Gao C
中科院分区：
文献类型：
--
作者：
Meng W;Zhang L;Cao M;Zhang Y;Zhang Y;Li P;Kang Z;Guo S;Xu P;Ma C;Gao C
溢流代谢引起的醋酸盐积累是制约各种细菌工业应用的主要问题。2,3-丁二醇（2,3- bd）在微生物中的合成是一个古老的代谢过程，其功能尚未明确。我们在这里证明了醋酸盐在阴沟肠杆菌亚种的生长过程中先增加后减少。dissolvens长效磺胺。双功能乙醛/乙醇脱氢酶adhe催化的乙醇生产和醋酸盐诱导的2,3- bd生物合成对于消除溢出代谢过程中产生的乙酸是必不可少的。葡萄糖的2,3- bd生物合成提供了通过adhe催化的乙醇生产消除乙酸所需的NADH。2,3- bd生物合成和乙醇生产的偶联策略在细菌中广泛存在，对消除有毒醋酸盐很重要。最后，我们实现了从几丁质中联合生产乙醇和乙酰胆碱，几丁质是第二丰富的天然生物聚合物，其分解代谢通过偶联乙酸消除策略不可避免地产生乙酸。像2,3- bd这样的无毒化学物质的合成可以看作是一种独特的具有理想代谢功能的溢出代谢。
Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then decreases during the growth of a bacterium Enterobacter cloacae subsp. dissolvens SDM. Both bifunctional acetaldehyde/ethanol dehydrogenase AdhE-catalyzed ethanol production and acetate-induced 2,3-BD biosynthesis are indispensable for the elimination of acetate generated during overflow metabolism. 2,3-BD biosynthesis from glucose supplies NADH required for acetate elimination via AdhE-catalyzed ethanol production. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and is important for toxic acetate elimination. Finally, we realized the co-production of ethanol and acetoin from chitin, the second most abundant natural biopolymer whose catabolism involves inevitable acetate production through the coupling acetate elimination strategy. The synthesis of a non-toxic chemical such as 2,3-BD may be viewed as a unique overflow metabolism with desirable metabolic functions.
登录
查看更多内容
DOI：
10.5423/ppj.oa.07.2013.0069
发表时间：
2013-12
期刊：
The plant pathology journal
影响因子：
--
作者：
Cho SM;Kim YH;Anderson AJ;Kim YC
通讯作者：
Kim YC
影响因子：
3.2
作者：
Fraedrich, Claudia;March, Anika;Haertig, Elisabeth
通讯作者：
Haertig, Elisabeth
DOI：
10.1073/pnas.220404397
发表时间：
2000-11-07
影响因子：
11.1
作者：
Loke, HK;Bennett, GN;Lindahl, PA
通讯作者：
Lindahl, PA
影响因子：
6.7
作者：
Thomas VC;Sadykov MR;Chaudhari SS;Jones J;Endres JL;Widhelm TJ;Ahn JS;Jawa RS;Zimmerman MC;Bayles KW
通讯作者：
Bayles KW
影响因子：
11.4
作者：
Songsiriritthigul, Chomphunuch;Lapboonrueng, Sasithorn;Yamabhai, Montarop
通讯作者：
Yamabhai, Montarop